Nihon Millipore Kabushiki Kaisha

Susumu Seto

David Wang

Takuji Kataoka

Atsumi Hirose, Deceased

A rapid, convenient, and highly sensitive method and kit for counting the number of living microorganisms are disclosed. A test solution is filtrated through a membrane filter element having a plurarity of hydrophilic sections substantially perfectly separated from each other by hydrophobic partitions. A microparticulate spray is then applied to said filter in order to add extracting and luminescence-inducing reagents in such an amount that allows the filter membrane within each section to become wet. Thereafter the sample obtained is processed by applying a highly sensitive bioluminescence image analyzing system thereto. The employment of a spray method for applying the extracting and luminescence-inducing reagents allows to substantially perfectly entrap the reagents and extracted substances within each of the hydrophilic sections, thus enabling, when combined with the use of a bioluminescence image analyzing system, to rapidly count the number without any or substantial requirement of cultivation.

Kit for counting the number of living microorganisms

A rapid, convenient, and highly sensitive method for counting the number of living microorganisms is disclosed, wherein a test solution is filtered through a membrane filter element having a plurarity of hydrophilic sections separated from each other by hydrophobic partitions. A microparticulate spray is then applied to the filter in order to add extracting and luminescence-inducing reagents in such an amount that allows the filter membrane within each section to become wet. Further, the sample obtained is processed by applying a highly sensitive luminescence image analyzing system thereto. The employment of a spray method for applying the extracting and luminescence-inducing reagents allows entrapment of the reagents and extracted substances within each of the hydrophilic sections. Therefore, when combined with the use of a luminescence image analyzing system, the number of bright spots so produced of which represent the living microorganisms may be rapidly counted without the requirement of cultivation.

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Kanagawa-ken, Japan

Susumu Seto