Kao Corporation

Susumu Ito

Shuji Kawai

Katsutoshi Ara

Katsuya Ozaki

Kazuaki Igarashi

Kikuhiko Okamoto

Yuji Hatada

Katsuhisa Saeki

Tohru Kobayashi

Shitsuw Shikata

Mikio Takaiwa

Moriyasu Murata

Eiichi Hoshino

Kenzo Koike

Kazushi Oshino

Akira Suzuki

Shigehito Adachi

Tadashi Yoshimatsu

Hiroshi Hagihara

Hiromi Kubota

Jun Hitomi

Michio Yokosuka

Yoshihiro Hakamada

Hirofumi Takigawa

Hiromi Okoshi

Yoko Watanabe

Hajime Mori

Yoshiaki Fujikura

Masaki Tsumadori

Shigeyoshi Tanaka

Hiroyuki Araki

Atsushi Tanaka

Yasuhiro Hayashi

Shigeo Inoue

Atsuo Nakae

Akira Takei

Yasunao Wada

Tomokazu Sato

Yuichi Ota

Yuichi Ohta

Masaharu Shimooka

Takaaki Uemura

Kaori Ikawa

Miyuki Kasai

Atsushi Suzumatsu

The present invention is directed to a liquefying alkaline alpha-amylase, and a DNA encoding for the same and functional fragments thereof.

Gene encoding alkaline liquefying &#x3b1;-amylase

The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.

Gene for enzyme having both alkaline pullulanase and alkaline alpha-amylase activities

Gene encoding alkaline liquifying alpha-amylase

DNA fragment containing gene for alkaline pullulanase

The invention relates to a mutant &agr;-amylase having an amino acid sequence obtained by making deletion or replacement by another arbitrary amino acid residue of at least a methionine residue at the 202-position or a position homologous thereto among amino acid residues set forth in SEQ ID NO:1, which constitute a liquefying alkaline &agr;-amylase, a gene thereof, and a detergent composition comprising the mutant &agr;-amylase. The mutant &agr;-amylase has the optimum pH in an alkaline range, an excellent &agr;-amylase activity, and high and lasting resistance to oxidizing agents, and is hence particularly useful as a component of detergent compositions containing a bleaching agent and an oxidizing agent.

Mutant &agr;-amylases

The present invention provides a DNA fragment encoding alkaline pullulanase exhibiting alkaline &agr;-amylase activity, alkaline &agr;-amylase possessing both an amino acid sequence described in SEQ ID NO:3 and a DNA fragment encoding the amylase, alkaline pullulanase possessing both an amino acid sequence described in SEQ ID NO:4 and a DNA fragment encoding the pullulanase, recombinant DNAs containing these DNA fragments, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting alkaline &agr;-amylase activity.

Gene for enzyme having both alkaline pullulanase and alkaline &agr;-amylase activities

Detergent compositions containing protopectinase whose optimum pH for the reaction is 7.0 or higher when protopectin or polygalacturonic acid is used as a substrate. The detergent compositions are endowed with remarkable detergency against mud soil.

Detergent composition

Liquefying alkaline .alpha.-amylase, process for producing the same, and

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for the microorganism Bacillus sp. Ferm BP-3376, which is capable of producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline-proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Bacillus SP. ferm BP-3376 and a method for its use to produce an

A biological pure culture of a microorganism belonging to the genus Rhodococcus and being capable of oxidizing the allyl position of .alpha.-cedrene, a process for producing sec-cedrenol comprising contacting a microorganism belonging to the genus Rhodococcus with .alpha.-cedrene, and a process for producing cedrenone comprising oxidizing sec-cedrenol produced by a microorganism belonging to the genus Rhodococcus, are disclosed. According to the process of the present invention, sec-cedrenol can be produced efficiently within a short period of time and with high purity. In addition, the process of the present invention can produce cedrenone with a high yield in a large quantity within a short period of time.

Microorganism belonging to the genus Rhodococcus, and process for

Process for the production of sec-cedrenol and cedrenone from

The present invention relates to a novel enzyme called alkaline proteinase K-16 that is characterized as having an optimum pH in an alkaline range, and stability in the presence of various surface active agents, such as sodium linear alkylbenzene sulfonate, sodium polyoxyethylene alkyl sulfate, sodium dodecyl sulfate, sodium .alpha.-olefin sulfonate, sodium alkyl sulfonate, and .alpha.-sulfo-fatty acid ester. The present invention further provides for a microorganism producing the novel alkaline proteinase, and a process for producing the novel alkaline proteinase. Alkaline proteinase K-16 of the present invention exhibits an excellent action against insoluble proteins and maintains sufficient activity over a wide temperature range and in the presence of surface active agents thus providing a use as an enzyme for detergents.

Alkaline proteinase isolated from bacillus sp.

An isolated alkaline pullulanase Y having .alpha.-amylase activity; a microorganism producing the alkaline pullulanase Y; and a process for producing the alkaline pullulanase Y are disclosed. The alkaline pullulanase Y having .alpha.-amylase activity has its optimum pH at higher alkaline range than conventional alkaline pullulanases and exhibits excellent stability in a wide pH range. Further, the alkaline pullulanase Y has strong resistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. Thus the alkaline pullulanase Y can advantageously be used as a detergent component.

Alkaline pullulanase Y having .alpha.-amylase activity

A novel alkaline pullulanase; a microorganism producing the alkaline pullulanase; and a process for producing the alkaline pullulanase are disclosed. The alkaline pullulanase of the present invention has a higher optimum pH range (pH 9.5-11) than conventional alkaline pullulanases and shows excellent stability in a wider pH range. It has also an optimum temperature of higher than 50.degree. C. and is thermally stable up to 40.degree. C. The alkaline pullulanase has also strong reistance to almost all detergent components such as surfactants, chelating agents, proteases for detergents, and the like. The alkaline pullulanase can advantageously be used as a detergent component. Also, it can be utilized for producing many kinds of oligosaccharides and also together with .alpha.-amylase for producing various monosaccharides.

Alkaline pullulanase from Bacillus sp. FERM P-10887

A mutant of an extracellular enzyme-producing microorganism belonging to genus Bacillus, which is resistant to a cell membrane synthesis inhibitor, including vancomycin and ristocetin, is disclosed. The cell membrane synthesis inhibitor-resistant mutant can produce such an enzyme as cellulase, protease, or amylase about 2 to 4 times of the parent strain. Such a mutant can be produced by subjecting an extracellular enzyme-producing microorganism tot a mutation treatment, and culturing said microorganism in a culture medium containing a cell membrane synthesis inhibitor.

Mutant resistant to cell membrane synthesis inhibitor and process for

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Utsunomiya, Japan

Susumu Ito