Novo Industri A/s

Susanne Rugh

Barrie E Norman

Poul Borge Poulsen

Erik Kjaer Markussen

Shmuel Amotz

Kurt Thomsen

Immobilized enzyme granules prepared by mixing inert filler particles into an aqueous solution or dispersion of a water soluble binder, followed by granulating the mixture so as to form carrier granules. Enzyme is immobilized on the hydrophilic surfaces of the carrier granules. If not already insoluble in the medium for the intended enzymatic reaction, the binder in the granules is rendered insoluble, e.g., water insoluble. Proteins are preferred binder materials, and glutaraldehyde is the preferred reagent for bonding enzyme to the carrier granules and for water insolubilizing the binder.

Carrier for immobilizing enzymes

Saccharification of low DP polysaccharides in syrups of high fructose content and low glucose content by short term contact with amyloglucosidase e.g. in less than 60 minutes, 1-10 AG units/gm of syrup solids, and syrup concentrations of 2-50 w/o solids in a continuous process employing immobilized AMG. Suitable high fructose content low glucose syrups are products that result from fractionation of isosyrup into enriched 50+% d.s.b. fructose syrups.

Saccharification of enriched fructose content syrups

Saccharification of low DP polysaccharides of high glucose content syrups by short term contact with amyloglucosidase e.g. 1-10 hours, 1-10 AG units/gm of syrup solids, and syrup concentrations of 5-25 w/o solids in a batch saccharification, less than 30 minutes in a continuous process employing immobilized AMG. Suitable high glucose content syrups are co-products that result from fractionation of isosyrup into 50+% d.s.b. fructose syrups and from production of crystalline dextrose.

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Vaerlose, Denmark

Susanne Rugh