Novo Nordisk A/s

Biogenerix Ag

Susann Taudte

Shawn DeFrees

David A Zopf

Walter Scott Willett

Henrik Clausen

Robert J Bayer

Caryn Bowe

Michael Felo

Matthew Kalo

Walter S Willett

The invention relates to methods of preparing and purifying conjugates between Granulocyte Colony Stimulating Factor and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are purified using various chromatography methods.

Glycopegylated granulocyte colony stimulating factor

The present invention provides conjugates between peptides and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.

One pot desialylation and glycopegylation of therapeutic peptides

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Pennsburg, PA, United States of America

Susann Taudte