E.i. Dupont De Nemours and Company

Alliance for Sustainable Energy, LLC

Nutrition & Biosciences USA 4, Inc.

Dupont Industrial Biosciences USA, LLC

Dupont Nutrition Biosciences Aps

International N&h Denmark Aps

Susan Marie Hennessey

Kevin D Nagy

Mark Scott Payne

Perry G Caimi

Robert DiCosimo

Richard T Elander

Richard Ray Bott

Ayrookaran J Poulose

Jayme L Paullin

T Joseph Dennes

Yougen Li

Melvin P Tucker, Iii

Ellen D Semke

Veli Alkan

Jared B Parker

Qiong Cheng

Jayarama K Shetty

Zheyong Yu

James B Dunson, Jr

Slavko Kralj

Yefim Brun

Robert Dicosimo

Ling Hua

Zhongmei Tang

Rodrigo Ramirez

Brian Douglas Mather

Hannu Koivikko

Stephen Thomas Breske

Charles R Powley

Dean M Fake

Tyler D Pritchett

Tapio Timo Viljava

Jyrki Kuusisto

Bogdan Szostek

Julie Friend

Negash Adugna

Michael Reichman

Erwin F Columbus-Hago

Erwin Columbus Hago

Juho Järvinen

Jennifer S Ovental

Wayne Atkinson

Mayis Seapan

Rahul B Kasat

Bonnie R Hames

James Gregory Wood

Mathias E Stolarski

Melvin Tucker

Daniel Eicholtz

Daniel Elcholtz

Bonnie Hames

Annemarie Mitchell

James B Dunson

Melvin P Tucker Iii

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Engineered glucosyltransferases

A method for producing insoluble alpha-1,3-glucan is disclosed. Embodiments of the method comprise providing (i) oligosaccharides that comprise alpha-1,3 and alpha-1,6 glycosidic linkages, or (ii) oligosaccharides derived from a glucosyltransferase reaction; and contacting at least water, sucrose, a glucosyltransferase enzyme, and the oligosaccharides provided in the first step. Glucosyltransferase reaction compositions embodying such a method, and insoluble products thereof, are also disclosed. Yield and other product benefits can be realized when practicing the disclosed subject matter.

Enzymatic production of alpha-1,3-glucan

Disclosed herein are aqueous compositions comprising fructose and methods of production thereof. A method for producing an aqueous composition comprising fructose can comprise, for example, conducting an enzymatic reaction by contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan having at least 30% alpha-1,3-linkages. A soluble fraction produced by such a reaction comprises at least about 55% fructose on a dry weight basis, and can be separated from insoluble poly alpha-1,3-glucan product(s), thereby providing an aqueous composition comprising fructose.

Production and composition of fructose syrup

Compositions comprising cellulase and at least one poly alpha-1,3-glucan ether compound having a degree of substitution with an organic group of about 0.05-3.0 are disclosed. Such compositions can be dry or aqueous, the latter of which can have a viscosity of at least about 10 cPs. The disclosed composition can be in the form of a personal care product, household product, or industrial product, for example. Also disclosed are a method for preparing an aqueous composition comprising cellulase and a poly alpha-1,3-glucan ether compound, and a method of treating a material such as fabric by contacting it with this aqueous composition.

Compositions containing one or more poly alpha-1,3-glucan ether compounds

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize alpha-glucan products having increased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

A method is disclosed for hydrolyzing an alpha-1,3 or alpha-1,6 glucosyl-glucose linkage in a saccharide (disaccharide or oligosaccharide). This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,3 or alpha-1,6 glucosyl-glucose linkage of the saccharide. This method is useful for reducing the amount of oligosaccharides in a filtrate isolated from a glucan synthesis reaction, for example.

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example.

Compositions comprising cellulase and at least one poly alpha-1,3-glucan ether compound having a degree of substitution with a positively charged organic group of about 0.05-3.0 are disclosed. Such compositions can be dry or aqueous, the latter of which can have a viscosity of at least about 10 cPs. The disclosed composition can be in the form of a personal care product, household product, or industrial product, for example. Also disclosed are a method for preparing an aqueous composition comprising cellulase and a poly alpha-1,3-glucan ether compound, and a method of treating a material such as fabric by contacting it with this aqueous composition.

Reaction solutions are disclosed herein comprising water, incompletely refined sucrose, and a glucosyltransferase enzyme that synthesizes insoluble poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages and a weight average degree of polymerization (DP) of at least 100. The yield of poly alpha-1,3-glucan by a reaction solution herein is at least 7% of the weight of sucrose that was converted in the reaction solution. Further disclosed are methods of producing poly alpha-1,3-glucan using incompletely refined sucrose, and poly alpha-1,3-glucan produced by these methods.

Production of glucan polymers from alternate sucrose sources

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Avondale, PA, United States of America

Susan Marie Hennessey