Dow Agrosciences LLC

Susan M Jayne

Manju Gupta

Terry R Wright

Sandeep Kumar

Diaa Alabed

Doug A Smith

Sara Bennett

Tristan E Coram

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentspecies,v1.

Maize ubiquitin promoters

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentgenotype,c.v. B104.

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentgenotype,c.v. Hi-II.

Thec.v. B73 Ubiquitin-1 (c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the samec.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a differentspecies,v2.

An inbred corn line, designated 4691436, the plants and seeds of the inbred corn line 4691436, methods for producing a corn plant, either inbred or hybrid, produced by crossing the inbred corn line 4691436 with itself or with another corn plant, and hybrid corn seeds and plants produced by crossing the inbred line 4691436 with another corn line or plant and to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic corn plants produced by that method. This invention also relates to inbred corn lines derived from inbred corn line 4691436, to methods for producing other inbred corn lines derived from inbred corn line 4691436 and to the inbred corn lines derived by the use of those methods.

Inbred corn line 4691436

Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from aUbiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

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Zionsville, IN, United States of America

Susan M Jayne