Dekalb Genetics Corporation

Susan J Zachwieja

David A Walters

Paul S Chomet

Paul C Anderson

Michael T Mann

Peggy G Lemaux

Thomas R Adams

Albert P Kausch

T Michael Spencer

William J Gordon-Kamm

James V O'Brien

Catherine J Mackey

Richard J Daines

Peter M Orr

Nancy G Willetts

Lucille B Laccetti

Thomas B Rice

William G Start

Michael A Stephens

Sheryl A Chambers

Mary L Mangano

Christopher E Flick

Donald S Walters

Emil M Orozco

Clayton S Vetsch

Kimberly Glassman

This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs thebar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof

Growing community of inventors

Waterford, CT, United States of America

Susan J Zachwieja