Eastman-kodak Company

Johnson & Johnson Clinical Diagnostics, Inc.

Clinical Diagnostic Systems, Inc.

Susan J Danielson

Ignazio S Ponticello

Richard C Sutton

Marsha D Oenick

David A Hilborn

Harold C Warren

Brent A Burdick

Michael W Sundberg

Barbara A Brummond

Michael R Detty

John Bruce Findlay

Donald P Specht

Fred T Oakes

Wayne A Bowman

Pranab Bagchi

Brian A Snyder

Daniel S Daniel

Margaret J Smith-Lewis

Glen M Dappen

Annie L Wu

Gregory J McClune

Karl J Sanford

Patricia M Scensny

Linda A Mauck

David F O'Brien

Paul H Frickey

Calvin Roman Messing

Allen L Thunberg

Susan B Littlehale

Patricia N Tyminski

Robert J Olyslager

A dry immunoassay analytical element, for assaying a ligand is disclosed. The element comprises, in the following order, (a) a layer containing a labeled ligand, (b) a bead spreading layer, c) a cross-linked hydrophilic polymer layer and d) a support; wherein (i) a fixed concentration of an immobilized receptor for the labeled ligand is located in a zone at the interface of layers (b) and (c); and (ii) the receptors are immobilized by being covalently bonded to polymeric beads that are smaller than the beads in layer (c).

Immunoassay elements having a receptor zone

A dry immunoassay analytical element, for assaying a ligand, comprising in the following order:

Immunoassay elements comprising polymers containing vandium IV (V+4) ions

What is disclosed is a dicarboxylic acid oxidation product of a immunologically reactive mono- or a polysaccharide having vicinal diols.

Dicarboxylic acid oxidation products

The invention is directed to labeled drug hapten analogues comprising:

Immunoassay with novel labeled drug hapten analogues

The invention is directed to an immunoassay for a thyronine derivative comprising:

Immunoassays with labeled thyronine hapten analogues

A biologically active reagent is prepared by reacting a biologically active substance covalently to a polymeric particle having pendant aldehyde groups. Such groups are provided by a polymerizable monomer represented by the structure: ##STR1## wherein Ar is arylene, R and R.sup.1 are independently hydrogen, halo or lower alkyl, R.sup.2 and R.sup.3 are independently alkylene of 1 to 4 carbon atoms in the chain, R.sup.4 is arylene, m is 0 or 1, and n is an integer of 1 to 4. These reagents can be used in a variety of specific binding and affinity purification methods, and in both solution assays and dry analytical elements.

Biologically active reagent prepared from aldehyde-containing polymer,

Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, such as enzymes, polypeptides and proteins. This attachment is carried out using carbamoylonium compounds which react with the carboxyl groups to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a carbamoylonium compound.

Attachment of compounds to polymeric particles using carbamoylonium

What is disclosed is a ligand analog comprising a dicarboxylic acid oxidation product of a immunologically reactive mono-or a polysaccharide having vicinal diols, to which a label or a support is appended through an amide or thioester linkage.

Ligand analogs for immunoassays derived from dicarboxylic acid oxidation

Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

Avidin-and biotin immobilized reagents and methods of use

Biologically active reagents are prepared from particles of copolymers having highly active succinimid groups. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through amide groups by displacement of highly active succinimid groups on the particle surface. These reagents are used to advantage in analytical elements, methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods such as affinity chromatography.

Method of preparing biologically active reagents from

Labeled drug hapten analogues for immunoassays

The invention is directed to new drug hapten analogues comprising:

Drug hapten analogues for immunoassays

Specific binding methods are used for diagnostic assays and purification separations whereby the specific binding capture reagent is prepared from copolymers having highly reactive carboxy groups. These groups are extended from the polymer surface with a linking group having from 8 to 50 atoms in the chain and two or more alkylene, arylene, alkylenearylene or arylenealkylene groups. To these reactive groups is attached a biologically active substance such as a protein or oligonucleotide which then participates in the diagnostic assays or purification separation methods.

Specific binding analytical and separation methods using carboxy

Water-insoluble, noncrosslinking, nonporous copolymers are provided which have recurring units derived from:

Succinimide containing polymers and lattices prepared from same

A water-insoluble immunoreactive reagent is prepared from a polymeric particle composed of a polymer derived from at least one ethylenically unsaturated polymerizable monomer having either pendant activated 2-substituted ethylsulfonyl or vinylsulfonyl groups. The interior of the particle is substantially free of detectable tracer material. The particle is covalently attached through the pendant groups to an immunological species which is capable of participating in an immunological reaction to complex with a corresponding receptor. This immunoreactive reagent can be incorporated in elements for use in immunoassays. In addition, they can be used in various immunological methods, including agglutination, sandwich and competitive binding assays where at least one immunological species is insolubilized.

Growing community of inventors

Rochester, NY, United States of America

Susan J Danielson