Life Technologies Corporation

Other

University of Houston System

Visigen Biotechnologies, Inc.

Susan H Hardin

Richard Coale Willson

Xiaolian Gao

James M Briggs

Shiao-Chun Tu

Keun Woo Lee

Ramin Homayouni

Paul Eric Hardin

Mitsu Reddy

Jun Ying

Nasanshargal Battulga

Andrei Volkov

Michael A Rea

Anelia Kraltcheva

Leslie Burgan Jones

Brent Mulder

Costa M Colbert

Chris Hebel

Ivan Pan

Alok Bandekar

Nucleotide triphosphate probes containing a molecular and/or atomic tag on a &#x3b3; and/or &#x3b2; phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.

Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Nucleotide triphosphate probes containing a molecular and/or atomic tag on a a &#x3b3; and/or &#x3b2; phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.

A method to be implemented on or in a computer is disclosed, where the method includes data collection, calibration, candidate selection, and analysis of data streams associated with each candidate to classify single molecule fluorescence resonance energy transfer events. Once classified, the classification can be related to the nature of the events, such as the identification of dNTP incorporation during primer extension to obtain a base read out of an unknown template.

Method for analyzing dynamic detectable events at the single molecule level

A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.

Methods for real-time single molecule sequence determination

Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity

A novel method of primer walking using octamer oligonucleotides to prime DNA sequencing reactions is described. Octamer sequencing is compatible with isotopic and fluorescent sequencing chemistry, reaction conditions are optimized such that the samples can be processed in parallel and the procedures are automated. This strategy is faster than the traditional primer walking sequencing strategy as the existence of a primer library allows immediate access to a primer for the next sequencing reaction, eliminating delays associated with designing and synthesizing gene specific primers. The octamer library is comprised of optimized sequencing primers, such that octamer sequencing yields results equivalent to or better than traditional primer walking.

Optimized primer library for gene sequencing and method of using same

Guanine-rich oligonucleotide primers and short telomeric DNA oligonucleotides that serve as non-conventional templates for the synthesis of long, nascent DNA strands are used to screen for pharmaceutical agents. Using the methods of the invention, agents exhibiting antagonistic or agonistic effects on guanine quartet formation can be selected for further study. The invention also provides a predictive assay for cancer in which the level of DNA polymerase activity at a quartet stabilized template is measured. The invention also contributes a method for screening pharmaceutical agents that reduce or inhibit DNA polymerase activity at guanine quartet stabilized templates. The invention provides a method of creating extended DNA molecules from oligonucleotide templates. The invention also provides a method of inhibiting the ageing process in which an oligonucleotide capable of forming non-Watson-Crick structures is used to promote the elongation of telomeres by DNA polymerase.

DNA replication templates stabilized by guanine quartets

Growing community of inventors

College Station, TX, United States of America

Susan H Hardin