Abbott Laboratories Corporation

Susan A Thacker

Nai-Yi Wang

Robert E Dubler

Frank S Ungemach

Roland L Walters

John A Walling

This disclosure related to a method and reagents for determining tetrahydrocannabinoids (THC) and THC metabolites in a biological fluid such as urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure for determining the presence of THC and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described also provides for novel wash reagent for a THC fluorescence polarization assay.

Fluorescence polarazation immunoassay for tetrahydrocannabinoids

Fluorescence polarization immunoassay for tetrahydrocannabinoids

Fluorescene polarization immunoassay for tetrahydrocannabinoids

A fluorescence polarization immunoassay for detecting the presence of methadone in a test sample is provided, based upon competition between methadone and a fluorescently labeled tracer for the binding sites on an antibody specific for methadone. The concentration of methadone in the sample determines the amount of tracer that binds to the antibody. The amount of tracer-antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of methadone in the sample. Tracers used as reagents and immunogens used to raise antibodies for use as reagents are also disclosed.

Methadone fluorescence polarization immunoassay

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Naperville, IL, United States of America

Susan A Thacker