Chemgenes Corporation

Brookhaven Science Associates, Lcc

Assoicated Universties, Inc.

Other

The United States of America As Represented by the United States

Ased, LLC

Snip Holdings, Incorporated

USA As Represented by Secretary of the Navy

The State University of New York

Oligos Etc. Inc.

Suresh C Srivastava

George E Meinken

Naveen P Srivastava

Ronnie Charles Mease

Sant K Srivastav

Leonard F Mausner

Powell Richards

Amy Yasko

Boris Leonidovich Zhuikov

Stanley J Szymanski, Jr

Satya P Bajpai

Gilbert R Gonzales

Andrei Laikhter

Vladimir Mikhailovich Kokhanyuk

Divya Pandey

Alok Kumar Srivastava

Radoslav R Adzic

Santhosh Kumar Thatikonda

Syed K Raza

Harold L Atkins

Stanislav Victorovich Ermolaev

Mark P Sweet

John W Babich

Jean-Francois Gestin

Rita Straub

Rashid A Fawwaz

Praveen K Shukla

Nicolai A Konyakhin

Dale P DeVore

Barry S Coller

Amy Arrow

Roger W Howell

Soldano Ferrone

Roderic M Dale

Thomas F Prach

Nikolay Alexandrovich Konyakhin

Stepan Vladimirovich Khamyanov

Natalya Roaldovna Togaeva

Saroj K Roy

Kathryn L Kolsky

Elena V Lapshina

Natalia R Togaeva

Satya K Bajpai

Kwok-Hung Sit

Satya P Bajpal

Zizhong Li

Wesley E Bolch

Provided herein are oligonucleotide probes for detecting 2019 novel coronavirus (2019-nCoV). The probes are modified at their 5' ends with a fluorophore (e.g., fluorescein), and are also modified (e.g., at their 3&#x2032; ends) with a moiety capable of quenching fluorescence from the fluorophore. The moiety is based on the IQ-4 or IQ-2 quencher. Also provided are kits including one or more of such oligonucleotide probes, and methods of detecting 2019-nCoV and/or diagnosing COVID-19 using the oligonucleotide probes and kits described herein.

RT-qPCR molecular detection and diagnosis

The present invention relates to novel process of reverse 5'&#x2192;3&#x2032; directed synthesis of RNA oligomers in the range of about 100-mer to about 200-mer has been developed and disclosed. Using that method demonstrated high quality RNA synthesis with coupling efficiency approaching 99%.

Highly efficient synthesis of long RNA using reverse direction approach

This invention is related to nucleic acid chemistry and describes novel 1,2-dithiolane functionalized nucleoside phosphoramidites (1, Chart 1) and corresponding solid supports (2, Chart 1). In addition to these derivatives, 1,2-dithiolane moiety can also be functionalized to at the various positions of the nucleobase and sugar part as shown in Schemes 1 to 8. The nucleosides of our invention carry a primary hydroxyl for DMTr (4,4'-dimethoxytrityl) function for chain elongation. Furthermore, the phosphoramidite function is attached at the 3&#x2032;-hydroxyl of the nucleoside. This allows oligonucleotide chain extension under standard DNA and RNA synthesis chemistry conditions and techniques, thus leading to high quality oligonucleotides. These derivatives are useful for introduction of reactive thiol groups either at 3&#x2032;- or 5&#x2032;-end of the oligonucleotides on the solid supports such as gold, silver and quantum dots.

Dithiolane functionalized nucleoside amidites and supports for stronger immobilization of bio-molecules on solid surfaces

This invention relates to novel method of synthesis of RNA utilizing N-2-acetyl protected guanine as nucleoside base, nucleosides, succinates, phosphoramidites, corresponding solid supports that are suitable for oligo deoxy nucleosides and RNA oligonucleotide synthesis. Our discovery using N-acetyl protected guanine as nucleoside base protecting group, which is significantly faster base labile protecting group, yet significantly more stable than commonly utilized-2-isobutyryl guanosine is a novel approach to obtain highest purity oligonucleotides. This approach is designed to lead to very high purity and very clean oligonucleotide, after efficient removal of the protecting groups, including acetyl group from guanine and to produce high purity therapeutic grade DNA oligonucleotides, RNA oligonucleotides, diagnostic DNA, diagnostic RNA for microarray platform. The deprotection of acetyl protecting groups of the natural deoxy and ribonucleosides occurs under substantially reduced time in contact with mild deprotection conditions such as mild bases, secondary amines for removal of such groups under such conditions would allows synthesis of various DNA and RNA of highest purity for diagnostics and therapeutic application. This approach is designed to lead to high purity large scale therapeutic grade oligonucleotide chimeras which consist of fluoro sugar modification in conjunction with deoxy nucleosides, ribonucleosides, modified base and modified sugar nucleosides. This approach is further designed to use acetyl guanine protecting group when other bases are sensitive nucleoside, and for use in oligo peptide synthesis and for support bound oligo nucleotides.

Synthesis of labile base protected-modified deoxy and modified ribo nucleosides, corresponding phosphoramidites and supports and their use in high purity oligonucleotide synthesis

The invention relates to nuclear technology, and to irradiation targets and their preparation. One embodiment of the present invention includes a method for preparation of a target containing intermetallic composition of antimony Ti&#x2014;Sb, Al&#x2014;Sb, Cu&#x2014;Sb, or Ni&#x2014;Sb in order to produce radionuclides (e.g., tin&#x2014;117m) with a beam of accelerated particles. The intermetallic compounds of antimony can be welded by means of diffusion welding to a copper backing cooled during irradiation on the beam of accelerated particles. Another target can be encapsulated into a shell made of metallic niobium, stainless steel, nickel or titanium cooled outside by water during irradiation. Titanium shell can be plated outside by nickel to avoid interaction with the cooling water.

Targets and methods for target preparation for radionuclide production

The present invention is directed to n-alkylated synthetic nucleosides of high regiospecific purity and oligonucleotides that can be utilized for studies on reversal of cytotoxic and mutagenic DNA damage, and as diagnostic tools.

Nucleosides and oligonucleotides for studies on reversal of cytotoxic and mutagenic damage of DNA and as diagnostics tools

Compositions and methods for treating and imaging vulnerable plaque and other inflammatory conditions in a patient rely on the delivery of conversion electron-emitting sources and other radionuclides to regions of exposed collagen in the vasculature or other body lumens. The conversion electron-emitting sources or other radionuclides are coupled to a collagen-binding substance and administered to the vasculature or other body lumen to permit binding for imaging and/or therapeutic purposes.

Methods and therapies for treating inflammatory conditions with exposed collagen

The present invention relates to novel phosphoramidites, A-n-bz, C-n-bz, C-n-ac, G-n-ac and U are produced with an HPLC purity of greater than 98% andP NMR purity greater than 99%. A novel process of reverse 5'&#x2192;3&#x2032; directed synthesis of RNA oligomers has been developed and disclosed. Using that method demonstrated high quality RNA synthesis with coupling efficiency approaching 99%.

RNA synthesis&#x2014;phosphoramidites for synthetic RNA in the reverse direction, and application in convenient introduction of ligands, chromophores and modifications of synthetic RNA at the 3'-end

The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides, and deuterated RNA's of defined sequences which can exhibit biochemically useful and biologically valuable properties, thus having potential for therapeutic uses.

Synthesis of deuterated ribo nucleosides, N-protected phosphoramidites and oligonucleotides

Synthesis of deuterated ribo nucleosides, N-protected phosphoramidites, and oligonucleotides

The thiol modified oligonucleotides have vast number of applications in the field of nucleic acid chemistry. The conjugates generated by mono thiol groups are unstable at higher temperature, in high salt concentration buffers and in presence other thiols. There is strong need to develop a novel thiol modifier probes that can generate multiple thiol groups. Described herein are efficient processes and compounds, dithiolane phosphoramidites derivative and dithiolane succinyl supports. The advantage of our cyclic disulfide thiol modifier is multifold a) each incorporation introduces two thiol groups; b) it can be introduced at any desired site of oligonucleotides; c) The symmetrical branching nature of the spacer in the linker arm of dithiolane allows for clean oligo synthesis, where cleavage of the linker arm and thereby of loss of oligo chain is prevented. We have successfully made 20-mer oligonucleotide containing single dithiolane derivative at 3', and 21-mer oligonucleotides containing single dithiolane derivative at 5&#x2032; or in the middle of the mixed base sequence. HPLC and ESI MS analysis of these oligonucleotides indicated satisfactory purity and correct composition of these oligos, respectively.

Dithiolane based thiol modifier for labeling and stronger immobilization of bio-molecules on solid surfaces

Synthesis of deuterated ribo nucleosides N-protected phosphoramidites, and oligonucleotides

This invention relates to synthesis of novel -N-FMOC protected nucleosides, succinates, phosphoramidites, corresponding solid supports that are suitable for oligo deoxy nucleosides and RNA oligonucleotide synthesis. Our discovery using N-FMOC as nucleoside base protecting group, which is highly base labile protecting group is a novel approach to obtain highest purity oligonucleotides. This approach is designed to lead to very high purity and very clean oligonucleotide, after efficient removal of the protecting groups and to produce high purity therapeutic grade DNA oligonucleotides, RNA oligonucleotides, diagnostic DNA, diagnostic RNA for microarray platform. The deprotection of FMOC protecting groups of the natural deoxy and ribonucleosides occurs under very mild deprotection conditions such as mild bases, secondary and tertiary amines for removal of such groups under such conditions would allows synthesis of various DNA and RNA of highest purity for diagnostics and therapeutic application. This approach is further designed to use FMOC protecting group on various base sensitive nucleoside, and for use in oligo peptide synthesis and for support bound oligo nucleotides. DNA oligonucleotides containing 3'-end dA at the 3&#x2032;-terminal will be produced using the FMOC-dA-supports would lead to much reduced M&#x2212;1 deletion sequences, and thereby high purity.

Synthesis of N-FMOC protected deoxy nucleosides, ribo nucleosides, modified deoxy and ribo nucleosides, and phosphoramidites, and their use in oligonucleotide synthesis

Growing community of inventors

Setauket, NY, United States of America

Suresh C Srivastava