Corning Incorporated

Becton Dickinson and Company Limited

Suparna Sanyal

Susan Xiuqi Qian

Elizabeth J Abraham

Deepa Saxena

In one aspect, there is provided a cell culturing substrate including:

Defined cell culturing surfaces and methods of use

In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.

A cell culture product is provided for propagating embryonic stem cells, and maintaining their self-renewal and pluripotency characteristics for extended periods of time in culture. The cell culturing product includes a substrate; and a coating thereon deposited from a coating solution. The coating solution includes a mixture of extracellular matrix proteins and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 &#x3bc;g/ml to about 1 mg/ml.

Extracellular matrix coated surface for culturing cells

A cell culture product is provided for propagating embryonic stem cells, and maintaining their self-renewal and pluripotency characteristics for extended periods of time in culture. The cell culturing, product includes a substrate; and a coating thereon deposited, from a coating solution. The coating solution includes a mixture of extracellular matrix proteins and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 &#x3bc;g/ml to about 1 mg/ml.

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Katy, TX, United States of America

Suparna Sanyal