Daesang Corporation

Suok Su Kim

Eun Seok Choi

Ji Ha Lee

Hyung Seop Youn

Tae Yong Kim

Hak Jun Kim

Seung Woo Cho

The present invention provides a novel allulose epimerase variant and various uses thereof, in which glycine (Gly), an amino acid residue at position 216 of the amino acid sequence of wild-type D-allulose 3-epimerase derived from, is substituted with serine (Ser). The novel allulose epimerase variant according to the present invention has a higher conversion activity of fructose to allulose compared to the wild-type D-allulose 3-epimerase derived from, and in particular, it has excellent thermal stability under high temperature conditions of 60° C. or higher, so that the enzyme conversion reaction is performed at the industrial level for mass production of allulose to prevent contamination, to shorten production time, and to reduce production cost.

Allulose epimerase variant, method of producing the same, and method of producing allulose using the same

The present invention provides: a novel allulose epimerase variant in which an amino acid residue present at a specific position of an amino acid sequence of a wild-type D-allulose 3-epimerase derived fromis substituted with another amino acid residue; and various uses of the novel allulose epimerase variant. The novel allulose epimerase variant according to the present invention has a higher conversion rate of fructose to allulose compared to that of the wild-type D-allulose 3-epimerase derived from, and has excellent thermal stability especially under high temperature conditions of 60&#xb0; C. or higher, and thus can prevent contamination during an industrial-scale enzymatic conversion reaction for the mass production of allulose, shorten production time, and reduce production costs.

Allulose epimerase variant, method for preparing the same, and method for preparing allulose using the same

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Yongin-si, South Korea

Suok Su Kim