Bristol-myers Squibb Compnay

Suo Win Liu

Thomas J Franceschini

Michael Politino

William V Burnett

Sean M Tonzi

Ross Binder

Joanne L Brown

Shu-Jen Chiang

Jonathan D Basch

Bi-cistronic plasmids used for the expression of formate dehydrogenase (FDH) and modified phenylalanine dehydrogenase (PDHmod) are provided.

Genetically stable plasmid expressing PDH and FDH enzymes

The invention provides a glutaryl 7-ACA amidase fromstrain BS-203, which catalyzes the hydrolysis of 7-&#x3b2;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.

Glutaryl cephalosporin amidase from Pseudomonas diminuta BS-203

The invention provides a glutaryl 7-ACA amidase from strain BS-203, which catalyzes the hydrolysis of 7-&bgr;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.

High expression vectors for expression of heterologous genes in Escherichia coli. The expression vectors contain, the tac promoter, an intergenic region, a restriction site, and, optionally, groES DNA.

High expression Escherichia coli expression vector

A D-amino acid oxidase promoter isolated from T. variabilis has been incorporated into S. cerevisiae expression vectors to increase the expression of heterologous genes. The heterologous promoter has been placed upstream of selective markers, auxotrophic nutritional and/or dominant selectable markers. Because the promoter is from another yeast specie, it is weakly recognized by and S. cerevisiae. To compensate for the weak transcripts of selectable marker genes transcribed from the weak heterologous promoter, the plasmid containing the gene of the interest is amplified leading to a higher expression of protein of interest.

Growing community of inventors

Longmont, CO, United States of America

Suo Win Liu