Council of Scientific and Industrial Research

Department of Biotechnology

Sunil Kumar Chattopadhyay

Sushil Kumar

Suman Preet Singh Khanuja

Mahendra Pandurang Darokar

Ram Prakash Sharma

Atul Prakash Kahol

Sachin Srivastava

Koneni Venkata Sashidhara

Vinayak Tripathi

Arvind Singh Negi

Suchitra Banerjee

Veena Prajapati

Ankur Garg

Asish Kumar Bhattacharya

Sudeep Tandon

Anirban Pal

Vijay Kumar Mehta

Arun Kumar Tripathi

Shipra Agarwal

Manish Kulshrestha

Ranganathan Santha Kumar Tirupadiripuliyur

Guru Das Bagchi

Arun Kumar Kukreja

Raghvendra Kumar Mishra

Prem Dutt Dwivedi

Vinayah Tripathi

Kunnath Padmanabhan Madhusudanan

Rajendra Prasad Patel

Tanpreet Kaur

The present invention provides a process for production of an anticancer taxoid brevifoliol of the formula I from plants belonging to the genusby first extracting the dried and pulverized leaves of the plant with an alcohol preferably at a temperature in the range of 20-40&#xb0; C. and then. concentrating the solvent to obtain an alcoholic extract. The alcoholic extract obtained is then adsorbed with an adsorbent and the resulting adsorbed material is then dried at a temperature ranging from 20-50&#xb0; C. for 4-48 hours. The dried adsorbed material is then extracted with a combination of an aliphatic solvent and a chlorinated solvent successively and concentrated to obtain a residue. The residue is subjected to gross fractionation using column chromatography such as silica gel, florosil and silicic acid followed by chromatography with a suitable adsorbent to get brevifoliol.

Process for preparing brevifoliol

The present invention relates to the bioactivity of taxanes isolated from the leaves of Himalayan Yew treeagainst human cancer cell lines grown in-vitro and subsequent identification of brevifoliol [1] as anticancer agent useful in the treatment of various types of cancer in humans 

Pharmaceutical composition containing brevifoliol for use in chemotherapeutic treatment of human beings, method therefor

Demethylation of 3', 4&#x2032;-dimethoxy or 3&#x2032;, 4&#x2032;, 5&#x2032;-trimethoxy benzoic ester of a phenol is carried out in the presence of an excess of aluminum halide in an organic solvent to get 4&#x2032;-Hydroxy, 3&#x2032;-methoxy or 4&#x2032; hydroxy, 3&#x2032;, 5&#x2032;-dimethoxy benzoic acid ester of a phenol. The reaction is also applicable to 3&#x2032;, 4&#x2032;, 5&#x2032;-trimethoxy diaryl ketone and some natural products like reserpine.

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Process for the production of vasicine

The invention provides a process for the production of a biologically active phenolic compound (&plus;) catechin from tissue cultures, said process comprising the steps of (a) inoculating the of explants on a culture medium supplemented with combinations of auxins and cytokinins, (b) incubating the cultures under continuous light or dark conditions for 4-6 weeks for callus initiation followed by subculturing at 4-6 weeks intervals, (c) extracting the fresh pulverized calli with polar solvents at room temperature; (d) evaporating the solvent to obtain a residue; and (e) treating the residue with a chlorinated solvent and isolating (&plus;) catechin compound by filtration.

Process for the production of a biologically active phenolic compound(&plus;) catechin

Synthetic precursors to 4-(P-methoxyphenyl)-2-amino-butane

Process for the preparation of 10-deacetylbaccatin III

A process has been developed for production of a food flavoring compound maltol which comprises (a) extracting the dried and pulverized leaves of the plants belonging to the genus Abies with an alcohol at 20-40&deg; C. and concentrating the solvent to obtain an alcoholic extract, (b) adsorbing the alcoholic extract with an adsorbent and drying the adsorbed material at a temperature ranging between 20-50&deg; C., (d) partitioning of the adsorbed material between selected solvents consisting of aliphatic hydrocarbon and chlorinated solvent successively, (d) concentrating the chlorinated solvent to a residue and crystallizing it from a suitable organic solvent or mixtures of such solvents to get pure maltol.

Process for the preparation of maltol from plants belonging to the genus Abies

The present invention relates to a process for the production of (&minus;)3,4-divanillyl tetrahydrofiran of formula (2) which comprises (a) isolating) (&minus;) secoisolariciresinol of formula (1) from the heartwood and roots of by an improved process which consists of partitioning of the alcoholic extract of the heartwood and roots of between water and chlorinated solvent, (b) extracting the chlorinated solvent extract with alkali and (c) isolating (&minus;) secoisolariciresinol from the alkali extract upon neutralization with mineral acid and extracting with organic solvent and (d) crystallizing it from suitable organic solvent, (e) dissolving the isolated (&minus;) secoisolariciresinol in suitable organic solvent and (f) reacting with triphenyl phosphine halide at 0-80&deg; C. for 1-10 hours and (g) isolating (&minus;) 3,4-divanillyl tetrahydrofuran by column chromatography.

Process for the production of (&minus;) 3,4-divanillyl tetrahydrofuran

This invention relates to a process for the production of a compound (&plus;) catechin penta acetate of formula I from tissue cultures which comprises : (a) inoculation of explants on different media compositions supplemented with combinations of auxins (1-5 mg/l) and cytokinins (0.1-1.0 mg/l); (b) incubation of the cultures under continuous light or dark conditions for 4-6 weeks for callus cultures followed by subculturing at 4-6 weeks intervals; (c) harvesting of the calli at different growth phases ranging from 12-36 months; (d) extraction of fresh pulverized calli with polar solvents at room temperature; (e) evaporating the solvent to give a residue; and partitioning of the residue between water and chlorinated solvents and evaporating the solvent to a semisolid mass; (f) subjecting the resultant mass to column chromatography over suitable adsorbent and (g) eluting with organic solvent/mixtures of organic solvents to get (&plus;) catechin penta acetate.

Process for the production of a compound (&plus;) catechin penta acetate useful as a precursor for the production of (&plus;) catechin

The invention provides a process for the preparation of 4-(p-methoxyphenyl)-2-aminobutane represented by structural formula (1) as shown herein below:

Process for preparing novel biologically active synthetic molecule 4-(p-methoxyphenyl)-2-amino-butane

The present invention provides a process for the production of two molecules of 4-aryl-2 butanols having the general formula 1 given below:

Process for the production of 4-aryl-2 butanols from Taxus wallichiana

The present invention relates to an improved process for the production of important taxol analogues 10-deacetyl taxol A,B and C of the formula (2) ##STR1## R=C.sub.6 H.sub.5 (10 deacetyl taxol A) =CH.sub.3 C.dbd.CHCH.sub.3 (10-deacetyl taxol B)

Process for the production of important taxol analogues 10-deacetyl

A process has been developed for production of taxols A, B, C with high yields from 7-xylosyl-10-deacetyl taxol A (taxol analogue A or, xyloside A), 7-xylosyl-10-deacetyl-taxol B (taxol analogue B or xyloside B), 7-xylosyl-10-deacetyl--taxol C (taxol analogue C or xyloside C), which comprises (i) isolating the taxol analogues A, B, C form the stembark of Taxus wallichiana by an improved process devoiding of solvent partitioning step, (ii) treating the isolated taxol analogues A, B, C with periodates in an acid free polar solvent medium to cleave the diol into dialdehyde at ambient temperature, (iii) reducing the dialdehyde solution with borohydride in a polar solvent--acetic acid medium at 0.degree.-40.degree. C. into an acetal, (iv) acidifying the resultant acetal with a mixture of mineral acid-polar solvent at 0.degree.-40.degree. C. into intermediate product 10-deacetyl taxols A, B, C, (V) reacting 10-deacetyl taxols A or B or C with a silane in presence of a base at 20.degree.-40.degree. C. to protect 2', 7-hydroxyl groups, of 10-deacetyl taxols A, B, C (vi) acetylating the 10-hydroxyl group in situ with an acetylating agent at 10.degree.-40.degree. C., (vii) deprotecting the 2', 7-hydroxyl groups with a mixture of mineral acid-polar solvent at 0.degree.-10.degree. C. (viii) isolating taxols A or B or C by chromatography over silica.

Process for the production of taxol

Growing community of inventors

Uttar Pradesh, India

Sunil Kumar Chattopadhyay