Genomictree, Inc.

Sungwhan An

TaeJeong Oh

Tae Jeong Oh

Myungsoon Kim

ChiWang Yoon

DaeKyoung Yoon

SunWoo Lee

SukKyung Woo

The present invention relates to a method of detecting methylation of an SDC2 gene, a composition for detecting methylation of an SDC2 gene, and a kit comprising same and, more particularly, to: a method of detecting methylation of an SDC2 gene by using primers for specifically amplifying a methylated SDC2 gene and a probe capable of complementarily hybridizing with a methylated SDC2 gene that has been specifically amplified by the primers; a composition for detecting methylation of an SDC2 gene; and a kit comprising same.

Method for detecting methylation of SDC2 gene

The present disclosure relates to a method of detecting methylation of target DNA in a multiplex manner and a composition for detecting methylation of target DNA, and more particularly to a method for detecting methylation of target DNA, comprising: constructing an oligonucleotide, which comprises a target-specific sequence capable of binding complementarily to the target DNA and a universal primer that does not bind complementarily to the target DNA; linearly amplifying the target DNA for linear target enrichment by using the oligonucleotide as a primer; and amplifying the linearly amplified target DNA using an oligonucleotide, which is capable of binding complementarily to the linearly amplified target DNA, a universal primer, and a probe.

Method for multiplex detection of methylated DNA

The present invention relates to the novel use of syndecan-2 (SDC2) gene as a CpG methylation biomarker for detecting precancerous lesions, and more particularly, to the use of the SDC2 gene as a biomarker to early diagnose colorectal precancerous lesions by measuring the degree of methylation of the SDC2 gene. The present invention provides a method of providing information for diagnosis of precancerous lesions by detecting methylation of the CpG islands of the SDC2 gene. In addition, the use of the methylation detection and kit according to the present invention makes it possible to diagnose colorectal precancerous lesions at an early transformation stage, thus enabling the early diagnosis of colorectal precancerous lesions. Furthermore, the methylation detection method and kit of the present invention enables colorectal precancerous lesions to be effectively diagnosed in an accurate and rapid manner compared to conventional methods.

Method for detecting precancerous lesions

A method for detecting the methylation of promoters using HpaII, a methylation-sensitive restriction enzyme. In such method, DNAs, derived from clinical samples or subjects to be diagnosed, are cut with HpaII, the cut DNAs are amplified by PCR with primers capable of amplifying CpG islands, and the presence or absence of the PCR amplification products is determined using a DNA chip for methylation detection. Unlike prior approaches, the inventive method allows the methylation of gene promoters to be detected in a simple and economical manner, and thus is useful for the diagnosis of diseases such as cancer that are characterized by methylation of gene promoters.

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Daejeon, South Korea

Sungwhan An