Panagene Inc.

Lg Chem-investment Ltd.

Sung-Kee Kim

Hyun-Ik Shin

Jay-Hyok Chang

Goon Ho Joe

Heekyung Park

Su Nam Kim

Se Ryun Kim

Kyoo-Woong Lee

Yongtae Kim

Do-Hyun Nam

Chwang Siek Park

Hyun-Il Lee

Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.

Melting curve analysis using PNA probe, method and kit for analyzing nucleotide polymorphism using melting curve analysis

Process for preparing a 1-substituted 5-hydroxymethyl imidazole

Growing community of inventors

Daejeon, South Korea

Sung-Kee Kim