Avellino Lab Usa, Inc.

Kt Corporation

Sun-Young Cho

Gene Lee

Yeong-Hwan Jeong

Connie Chao-Shern

Yong-Beom Yi

Zu-Cheul Lee

Won-Yong Kim

Andrew Nesbit

Larry DeDionisio

Tara Moore

Methods and compositions for the treatment of a corneal dystrophy in a subject in need thereof are provided. In one aspect, the method includes the step of obtaining a plurality of stem cells comprising a nucleic acid mutation in a corneal dystrophy target nucleic acid from the subject and manipulating the nucleic acid mutation in one or more stem cells of the plurality of stem cells to correct the nucleic acid mutation, thereby forming one or more manipulated stem cells. The manipulated stem cells are isolated and then transplanted into the subject. In some embodiments, the nucleic acid mutation is manipulated using CRISPR system.

Methods for the treatment of corneal dystrophies

Systems and methods for detecting at least two genomic alleles associated with corneal dystrophy in a sample from a human subject are disclosed in which cells (e.g., epithelial) of the subject are adhered to a tip of a substrate. The tip of the substrate is agitated in a lysis solution that lyses cells adhered to the substrate. The substrate is removed from the lysis solution upon completion of this agitation. The resulting lysis solution is incubated and then genomic DNA from the lysis solution is isolated to form a gDNA solution. From this, identity of at least two nucleotides present in the human TGF&#x3b2;I gene is determined using at least two oligonucleotide primer pairs and the gDNA solution. These at least two nucleotides are located at respective independent positions of the TGF&#x3b2;I gene corresponding to respective independent single nucleotide polymorphisms (SNPs) associated with corneal dystrophy.

Methods for multiplex detection of alleles associated with ophthalmic conditions

Provided is an apparatus may be provided for transmitting a moving image including an alpha channel. The apparatus may include an object region extractor configured to extract an object region from the moving image, a color frame generator configured to generate a color frame corresponding to the extracted object region, an alpha channel frame generator configured to generate an alpha channel frame corresponding to the object region based on the alpha channel included in the moving image, a synthesizer configured to generate a synthesized frame by synthesizing the color frame and the alpha channel frame; an encoder configured to encode the synthesized frame; and a transmitter configured to transmit the encoded synthesized frame to a reproducing device.

Video transmitting device and video playing device

Growing community of inventors

Seoul, South Korea

Sun-Young Cho