Korea Advanced Institute of Science and Technology

Intelligent Synthetic Biology Center

Korea Research Institute of Chemical Technology

Other

Konkuk University Industrial Cooperation Corp

Samyang Genex Co.

Sun-Chang Kim

Bong Hyun Sung

Hyun-soo Lee

Seung-Suh Hong

Ju Young Lee

Jong Geon Jegal

Jun Hyoung Lee

Young Woong Lee

Ki Jung Lim

Chan-Bae Park

In Seung Jang

Seohyun Kim

Ji-Hyun Park

Da Jung Kim

Woo Hyun Kim

Sang-Hee Lee

Jung-Kul Lee

Wan Taek Im

Woohyun Kim

Byung-Kwan Cho

Ju Ri Shin

Jae Hyun Lee

Yeon Hwa Lee

Suk Chae Jung

Su A Jang

Tae-Su Kim

Min Hyung Kang

Wan-Taek Im

Gil Tsu Choi

Jeong Hyun Kim

Myung Keun Park

In-Cheol Kim

Jae-Hyun Lee

Dong-Kyun Lee

Ju-Hyun Cho

Da-Jung Kim

In-Yup Park

Kui-Hyeon Kang

Donghui Choe

Suk Chea Jung

Sungjoo Park

Eun Su Ko

Ranjitha Ramakrishnan

Giltsu Choi

Suk-Chae Jung

Soohwan Lim

Kyung Seok Yang

Su-A Jang

Ki-Jung Lim

Ju-Ri Shin

Ju-Young Lee

Bong-Hyun Sung

Jun-Hyoung Lee

Byung-Jo Yu

Provided is a method of producing a novel microorganism having a minimal genome. In particular, provided are a method of producing a novel microorganism having a smaller genome size than a wild-type, the method including the step of culturing the microorganism in a medium containing LB broth with stepwise decreasing concentrations of the LB broth; and a novel microorganism having a smaller genome size than a wild-type, produced by the above method. Despite the small genome size, the novel microorganism of the present disclosure may have a high growth rate, pyruvate production capacity, and protein translation efficiency thereby being usefully applied to industry such as production of recombinant proteins or pyruvate.

Microorganism having minimal genome and method of producing the same

Provided are a recombinant yeast having improved ability to produce ginsenoside, which is prepared by overexpressing INO2 and INO4 or deleting OPT1 in a yeast having ability to produce ginsenoside, a method of preparing the yeast, and a method of producing ginsenoside by using the yeast.

Increased production of ginsenosides through yeast cell organelle improvement

The present invention relates to recombinant yeast, in which the productivity of ginsenoside is enhanced by overexpressing CPR5, PDI1, or ERO1 in yeast having the productivity of ginsenosides; a method for preparing the yeast; and a method for producing ginsenosides using the yeast.

Increased production of ginsenosides through improvement of protein-folding machinery of yeast

The present invention provides an antimicrobial peptide polymer comprising at least one monomer which is digested by pepsin, a multimeric antimicrobial peptide complex comprising the polymer and a cell surface anchoring motif linked to the polymer, an antimicrobial microorganism displaying the multimeric antimicrobial peptide complex, an antimicrobial composition comprising the same, a method of treating an infectious disease caused by bacteria, yeast or fungi by administering the antimicrobial composition, and a method for producing the antimicrobial microorganism. According to the invention, living microorganisms displaying an antimicrobial peptide on the cell surface thereof may be administered in vivo without having to lyse the microbial cell and isolate and purify the antimicrobial peptide, so that the antimicrobial peptide exhibits antimicrobial activity. Thus, the antimicrobial peptide may be produced at significantly reduced costs so that it may have widespread use.

Multimeric antimicrobial peptide complex which is displayed on cell surface

The present invention relates to a composition for wound healing or skin regeneration comprising gypenoside 75 (gypenoside LXXV) as an active ingredient.

Composition for treating wound comprising gypenoside LXXV

This invention relates to a novel formaldehyde dehydrogenase expressed by a formaldehyde dehydrogenase gene and having independent reduction activity for formic acid, a method of preparing the formaldehyde dehydrogenase from a strain transformed with a recombinant expression vector including the gene, and a method of producing formaldehyde from formic acid through a reduction reaction of the formaldehyde dehydrogenase.

Formaldehyde dehydrogenase and method for preparing formaldehyde using same

Provided are a novel UDP-glycosyltransferase (uridine diphosphate glycosyltransferase) protein having glycosyltransfer activity for glucose linked by a glycosidic bond at the C-20 position of PPD (protopanaxadiol)-type or PPT (protopanaxatriol)-type ginsenoside, and use thereof.

Glycosyltransferase derived from Dolwoe and use thereof

The present invention relates to a novel uridine diphosphate (UDP)-glycosyltransferase, and particularly to a novel UDP-glycosyltransferase derived fromand use thereof, a method for preparing glycosylated ginsenoside by converting protopanaxadiol (PPD)-type ginsenoside using the UDP-glycosyltransferase, a composition for converting the PPD-type ginsenoside into glycosylated ginsenoside, comprising the UDP-glycosyltransferase, a transformant or a culture thereof as active ingredients, a method for enhancing the expression of the UDP-glycosyltransferase using MeJA (methyl jasmonate), and a composition for enhancing the expression of the UDP-glycosyltransferase, which comprises MeJA as an active ingredient.

UDP-glycosyltransferase derived from ginseng and use thereof

Disclosed are an amphipathic peptide-lipase conjugate with enhanced lipase activity, a polynucleotide coding for the conjugate, an expression vector carrying the polynucleotide, a transformant anchoring the expression vector therein, a method for preparing the conjugate, a lipolysis method using the conjugate, and a method for producing biodiesel using the lipase.

Amphipathic peptide-lipase conjugate having advanced lipase activity and use thereof

Disclosed are novel antimicrobial peptides which can promote the regeneration of skin cells, thus healing wounds. Pharmaceutical compositions comprising the peptides as active ingredients are also provided for wound healing and skin rejuvenation. The antimicrobial peptides exhibit inhibitory activity against antibiotic-resistant strains, and their antimicrobial activity is maintained without loss of structural stability even under a high salt condition. Also, being proven to promote the migration and regeneration of skin cells in mice as well as in vitro, the antimicrobial peptides may be widely used as an agent for regenerating skin cells. Further, they can find applications in various fields including the medical industry and the cosmetic industry. Hence, the novel antimicrobial peptides are anticipated to have considerable repercussions in the market for antibiotics, wound healing agents and cosmetics.

Use of antimicrobial peptides in regeneration of skin cells

The present invention relates to an artificial transcription factor which can artificially regulate gene expression of an, wherein the transcription factor comprising zinc finger proteins and transcription factors of prokaryote, and to be engineeredusing the same. Specifically, the artificial transcription factors comprising zinc finger domains and transcription factors inas effector domains are prepared and said artificial transcription library is introduced toto effectively and artificially regulate gene expression regardless of an activity of endogenous transcription factors in theand to inducehaving various desired phenotypes. Thus, onlyhaving the desired phenotypes useful for industries can be selected and used.

Preparation of an artificial transcription factor comprising zinc finger protein and transcription factor of prokaryote, and a use thereof

Disclosed is a method for developing novel strains deleted specific chromosome sites, using transposon and Cre/loxP site-specific recombination by Cre expression vector, wherein the transposon comprises a selectable marker and loxP site. The method comprises the steps of: (1) preparing a transposon comprising a selectable marker and loxP site; (2) inserting the transposon into an optional position of microbial chromosome, and determining the inserted site; (3) integrating two transposons comprising a different selectable marker to one chromosome; (4) deleting a chromosomal site between the two lox sites by introducing a Cre expression vector into the chromosome of step (3); and (5) repeating steps (3 and 4) for the mutant deleted a part of chromosome, to shorten the chromosome of mutant gradually.

Genome minimization by tn5-coupled cre/loxP excision system

Disclosed is a novel antimicrobial peptide having excellent antimicrobial activities, its analogs and an antimicrobial composition comprising them. The antimicrobial peptide alternatively comprises basic amino acid residues and hydrophobic amino acid residues, and is able to penetrate into microbial cells and act against a wide variety of microorganisms.

Antimicrobial peptide, its analogs and antimicrobial composition comprising them

The present invention relates to peptides that are more potent than or equally potent as the conventional antimicrobial peptides and has strong antimicrobial activities at high salt concentration.

Peptides having biological activity

The present invention provides a method for mass production of an antimicrobial peptide, which comprises the steps of: constructing a fusion gene containing a first gene encoding a negatively charged acidic peptide having at least two cysteine residues and a second gene encoding a positively charged basic antimicrobial peptide; transforming a host microorganism with an expression vector comprising the fusion gene; cultivating the transformed microorganism to express a fusion peptide containing the acidic peptide and antimicrobial peptide, and, recovering the expressed antimicrobial peptide. In accordance with the present invention, the inhibitory effect of the expressed antimicrobial peptide on the growth of the host microorganism can be dramatically minimized by fusing it with the acidic peptide. Accordingly, antimicrobial peptides can be produced massively from a recombinant microorganism regardless of the kind of the antimicrobial peptides.

Method for mass production of antimicrobial peptide

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Daejeon, South Korea

Sun-Chang Kim