Pfizer Corporation

Maxygen, Inc.

Pfizer Products Inc.

Codexis Mayflower Holdings, LLC

Maxygar, Inc.

Sun Ai Raillard

Jeremy S Minshull

Claes Gustafsson

Willem P C Stemmer

Yan Chen

Anke Krebber

Kim Jonelle Stutzman-Engwall

Phillip A Patten

Brian S Carr

Lorraine Joan Giver

Mark D Welch

Robin A Emig

Steven Henry Bass

Matthew B Tobin

Andreas Crameri

Joseph A Affholter

Stanley Goldman

Pascal Longchamp

Seran Kim

Gjalt W Huisman

Stephane J Jenne

Stephan Jenne

Yong Hong Chen

S Christopher Davis

Claus M Krebber

Simon Christopher Davis

Claus Gustafsson

Venkitswaran Subramanian

Jeremy Stephen Minshull

Claes Eric Gustafsson

Claes Eric Daniel Gustafsson

Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.

Integrated systems and methods for diversity generation and screening

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of. The present invention further relates to vectors, host cells, and mutant strains ofin which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

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Integrated system for diversity generation and screening

Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.

High throughput mass spectrometry

The invention provides methods for generating, identifying, and selecting polynucleotides encoding novel starch metabolizing enzymes (NSME), NSME-encoding polynucleotides, compositions of recombinant shuffled NSME protein, plant cells and microbes containing a shuffled NSME polynucleotide in expressible form, plants containing a shuffled NSME polynucleotide in expressible form, novel starch compositions produced by plants and cells, uses of such plants, cells, and starch compositions.

Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes

The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of . The present invention further relates to vectors, host cells, and mutant strains of in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.

Directing the ratio of B2:B1 avermectins in Streptomyces avermitilis host cells

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Mountain View, CA, United States of America

Sun Ai Raillard