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Sumona Dhara

Kapil Bardeja

Nitin S Kale

Dadasaheb Sangave

An asymmetric immobilization process that immobilizes a one or more receptor biomolecules on one or more solid surfaces is provided. The asymmetric immobilization process includes the following steps: (i) activating the one or more solid surfaces using an oxidizing agent; (ii) treating the one or more solid surfaces with aminosilane to obtain a one or more amine functionalized solid surfaces; (iii) treating the one or more receptor biomolecules with a 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) to obtain a one or more site-specific EDC activated receptor biomolecules; and (iv) immobilizing the one or more site-specific EDC activated receptor biomolecules on a first layer of the one or more amine functionalized solid surfaces, wherein the immobilizing comprises treating the one or more site-specific EDC activated receptor biomolecules with amine groups of the first layer of the one or more amine functionalized solid surfaces to form a covalent amide bond.

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Thane, India

Sumona Dhara