Nuclera Ltd

Sumit Kalsi

Gordon R McInroy

Michael Chun Hao Chen

Richard J Paolini, Jr

Luke M Slominski

Cristina Visani

David Zhitomirsky

Christopher Dulson

Sihong Chen

Stephanie Reikine

Laurence Livingstone Bell

Rick Paolini

Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

Methods for cell-free protein expression

A method for moving an aqueous droplet comprising providing an electrokinetic device including a first substrate having a matrix of electrodes, wherein each of the matrix electrodes is coupled to a thin film transistor, and wherein the matrix electrodes are overcoated with a functional coating comprising: a dielectric layer in contact with the matrix electrodes, a conformal layer in contact with the dielectric layer, and a hydrophobic layer in contact with the confornial layer; a second substrate comprising a top electrode; a spacer disposed between the first substrate and the second substrate and defining an electrokinetic workspace; and a voltage source operatively coupled to the niatrix electrodes. The method further comprises disposing an aqueous droplet on a first matrix electrode; and providing a differential electrical potential between the first matrix electrode and a second matrix electrode with the voltage source, thereby moving the aqueous droplet.

Method of electrowetting

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Cambridge, United Kingdom

Sumit Kalsi