The United States of America, As Represented by the Secretary, Department of Health and Human Services

Lyell Immunopharma, Inc.

Suman Kumar Vodnala

Nicholas P Restifo

Robert L Eil

Rigel J Kishton

Robert Langland Eil

Provided are methods of producing an isolated population of T cells, the method comprising culturing isolated T cells in vitro in the presence of hydroxycitric acid, and/or a salt thereof, wherein the salt is potassium hydroxycitrate or sodium hydroxycitrate. Also provided are related isolated populations of cells, pharmaceutical compositions, and methods of treating or preventing cancer in a mammal.

Methods of producing T cell populations using hydroxycitric acid and/or a salt thereof

The preset disclosure provides methods of culturing cells, e.g., pluripotent cells, multipotent cells, and/or immune cells (e.g., T cells, NK cells, and/or TILs) in a medium comprising at least about 5 mM potassium ion, wherein the medium is not hypertonic. In some aspects, the medium is hypotonic. In some aspects, the methods disclosed herein increases the number of less-differentiated cells in the population of cells. In some aspects, the cultured cells are engineered, e.g., to comprise a chimeric antigen receptor or an engineered T cell receptor. In some aspects, the cells are administered to a subject in need thereof.

Growing community of inventors

San Mateo, CA, United States of America

Suman Kumar Vodnala