Biocon Limited

Suma Sreenivas

Nagaraj Govindappa

Kedamath Nanjund Sastry

Kedarnath Nanjund Sastry

Harish Iyer

Partha Hazra

Yogesh Basavaraju

Manjunath Hadavanahalli Shivarudraiah

Komal N Kanojia

Shivakumar Madenahalli Channabasappa

Srikanth Gollarahosahalli Sathyanarayana

Sankar Periyasamy

Srividya Divakar

The present disclosure relates to a process of identification and isolation of sorbitol dehydrogenase promoter from. Further, the present disclosure also relates to expression of heterologous proteins under the control of Sorbitol dehydrogenase promoter in

Nucleotide sequence and a process thereof

The disclosure relates to a process of obtaining a fully folded two chain insulin glargine that require no further processing to make it functionally active. The present disclosure discloses a surprising effect of over expression of Kex2p intracellularly under the control of inducible FLD1 promoter in the hostto produce two chain functional glargine secreted directly in the medium. The schematic diagram of how the two chains are made inside the hostand secretes into the medium.

Process of expressing a fully folded functional two chain insulin glargine

The present invention relates to the preparation of insulin compounds including their analogs or derivatives thereof from their corresponding precursor forms by a one step enzymatic reaction involving the combinatorial and concurrent use of optimal quantities of trypsin and carboxypeptidase B that work synergistically directing the reaction in a controlled manner to avoid production of random undesired byproducts. Particularly, the enzymatic conversion reactions of the instant invention offer advantages of reduction in the number operational steps, higher yield and purity of the desired end products.

Process for preparation of insulin compounds

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Bengaluru, India

Suma Sreenivas