10X Genomics, Inc.

Sultan Doganay Tuncer

Augusto Manuel Tentori

Hanyoup Kim

Yifeng Yin

Eswar Prasad Ramachandran Iyer

David Michael Patterson

Shea Thompson Lance

Meghan L F Frey

Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification. For example, provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample where a splint oligonucleotide hybridizes to a second strand; the second strand is ligated together thereby creating a circularized second strand, rolling circle amplification of the circularized second strand results in generation of an amplified second strand, and all or part of the sequence of the amplified second strand is determined and used to spatially detect the analyte in the biological sample.

Methods for spatial analysis using rolling circle amplification and detection probes

Growing community of inventors

Pleasanton, CA, United States of America

Sultan Doganay Tuncer