Sungkyunkwan University

Suk-Tae Kwon

Mi-Sun Lee

Jeong Jin Choi

Jung-Hyun Lee

Gun-A Kim

Ki Hoon Nam

Disclosed are a method of preparing an activeB-type DNA polymerase (Neq DNA polymerase), an active Neq DNA polymerase prepared according to the method, and a polymerase chain reaction (PCR) using the active Neq DNA polymerase. The active Neq DNA polymerase may be used in various nucleic acid polymerization reactions, such as PCR.

Method for preparing active nanoarchaeum equitans DNA polymerase and the active DNA polymerase prepared by the method

The present invention provides uracil-DNA glycosylase (UDG) gene originating fromsp. HJ147, and amino acid sequences deduced from the gene; expression and purification of Psp HJ147 UDG gene in; and characterization of UDG obtained therefrom, and the use thereof in a polymerase chain reaction (PCR). The UDG according to the present invention has a specific activity of excising uracil bases in a uracil-containing DNA substrates at a low temperature, and is easily heat-inactivated. It thus can effectively eliminate cross contamination and carry-over contamination of PCR templates often occurring after a PCR process using dUTP. Therefore, it is useful for increasing preciseness (elimination of false positives), purity and amplification efficiency of PCR.

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Gyeonggi-do, South Korea

Suk-Tae Kwon