Korea Research Institute of Bioscience

Korea Research Institute of Bioscience and Biotechnology

Suk Ran Yoon

In Pyo Choi

Sooyun Lee

Han Na Kim

Soo Yeon Park

Sol Ji Jung

The present disclosure relates to a method for producing of a memory-like natural killer cell having an ability to produce a higher level of a natural killer cell receptor, having a better killing capacity, and having an ability to produce a higher level of IFN-γ than a natural killer cell in human peripheral blood, and a memory-like natural killer cell produced by the method, and a cancer treatment method using the memory-like natural killer cell.

Method for producing memory-like NK cells with ability to express higher levels of NCRs, cytotoxicity, and IFN-γ than NK cells in human peripheral blood

Disclosed is a method for producing a large amount of natural killer cells and the use of the natural killer cells as an anticancer agent. The method produces fresh NK cells with high purity within a short time, and can also produce cold-preserved NK cells and thawed cryopreserved NK cells having efficacy comparable to the fresh NK cells. NK cells having efficacy comparable to the fresh NK cells can also be produced from cryopreserved CD3-negative cells. The fresh NK cells, cold-preserved NK cells and cryopreserved NK cells exhibit therapeutic effects against various cancers, including colorectal cancer, lung cancer, liver cancer, pancreatic cancer and leukemia, indicating these NK cells are effective as cellular therapeutic agents. Also disclosed are doses and methods of administration that show excellent effects when the fresh NK cells, cold-preserved NK cells and cryopreserved NK cells are used as pharmaceutical compositions for cellular therapy.

Method for mass producing natural killer cell and use of natural killer cell obtained by the method as anti-cancer agent

Growing community of inventors

Daejeon, South Korea

Suk Ran Yoon