Henan Zhongda Hengyuan Biotechnology Stock Co., Ltd.

Sujing Gu

Linzheng Li

Ziheng Jin

Yanjun Wen

A method of preparing aseed extract rich in anthraquinones and a galactomannan extract includes the following steps: (1) crushingseeds into aseed powder; (2) extracting theseed powder with 40-85% ethanol, filtering to obtain an extract solution and a residue; (3) concentrating the extract solution under vacuum to obtain a concentrated extract solution, spray-drying the concentrated extract solution to obtain theseed extract; (4) extracting the residue with membrane filtered water, conducting a centrifugation to obtain a supernatant; (5) adding ammonium sulfate and ethanol to the supernatant to form a two-phase aqueous system, collecting a bottom layer of the two-phase aqueous system; and (6) conducting an ultrafiltration of the bottom layer with a cut-off molecular weight of 50 k-200 k to obtain a galactomannan extract solution, drying the galactomannan extract solution under vacuum to obtain the galactomannan extract.

seed extract and a method for comprehensive development and utilization ofseeds

A method for preparing aThunb extract rich in dihydromyricetin includes the following steps: (1) crushingThunb seeds to obtain aThunb powder; (2) adding a 10-95% ethanol solution in an amount of 3-15 times of an amount of theThunb powder, stirring and extracting at 20&#xb0; C.-80&#xb0; C. twice; (3) filtering to obtain an extract solution; (4) concentrating the extract solution by evaporating ethanol under reduced pressure to obtain a crude extract, the crude extract having a solid content of 10%-40%; (5) placing the crude extract at &#x2212;20&#xb0; C. to 8&#xb0; C. for 0.5 to 12 hours; (6) centrifuging the crude extract to obtain a supernatant; and (7) spray-drying the supernatant to obtain theThunb extract.

Method of preparing Hovenia dulcis Thunb extract rich in dihydromyricetin

A method of preparing aflower extract rich in isoflavones includes: grinding dryflowers to obtain aflower powder; adding a first 20-50% ethanol solution to form a mixture, stirring and heating the mixture at 20-80&#xb0; C. for 1-4 hours, removing the first solution, adding a second 20-50% ethanol solution, stirring and heating the mixture, removing the second solution, and combining the first solution and the second solution to obtain an extract solution; filtering the extract solution to remove solids; concentrating the extract solution under vacuum to obtain a crude extract containing 10-40% solid components; adding anhydrous ethanol solution to reach an ethanol concentration of the crude extract of 75-95%, storing the crude extract at 20-30&#xb0; C. for 1-4 hours; centrifuging the crude extract at a speed of 2,000-5,000 r/min to obtain a supernatant; and concentrating and spray drying to obtain theflower extract without any purification steps.

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Luohe, China

Sujing Gu