Tecan Trading Ag

Becton Dickinson and Company Limited

Sujata Iyer

Patrick D Kinney

Tiffany Ding (Lay)

Tin Ngo

Philipp Ott

Adrian Sager

Jennifer Ji

Joseph T Trotter

Manjeet Dhindsa

Marta Matvienko

Daniel Tran

Daniel Hoffmeyer

John F Dunne

Michael Keller

Douglas Petry

Hagen Reinhardt

Torleif Ove Björnson

Tiffany Ding

A pipette tip extension having a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end is disclosed. The exterior wall has an outer side and an inner side and encloses an inner cavity which is delimited by the inner side of the exterior wall. The exterior wall forms at the proximal end a reception aperture. The pipette tip extension further has one or more distance elements arranged at the inner side of the exterior wall and protruding into the inner cavity.

Pipette tip extension, pipette tip, assembly of pipette tip and pipette tip extension, and method of use

A method of treating an isolated area of a sample with a liquid is disclosed. The method has the steps of generating an isolated area of a sample by a first fluid reservoir where the first fluid reservoir enclosing a distal end of a second fluid reservoir and the isolated area of the sample being sealed towards the remaining area of the sample, bringing the isolated area of the sample into fluid connection with the second fluid reservoir, dispensing a fluid from the second fluid reservoir into the first fluid reservoir thereby generating a fluid flow on the sample at the isolated area in a first direction, and aspirating the fluid from the first fluid reservoir into the second fluid reservoir thereby generating a fluid flow on the sample at the isolated area in a second direction.

Method of treating a sample

A cartridge, in particular a disposable cartridge, for use in an electrowetting sample processing system. The cartridge contains an internal gap with at least one hydrophobic surface for enabling an electrowetting induced movement of a microfluidic droplet that has magnetic beads and further has a bead accumulation zone, into which the microfluidic droplet is transferable by electrowetting force and the magnetic beads are exposable to a magnetic force of a bead manipulation magnet. The internal gap has a bead extraction opening adjacent to the bead accumulation zone. The bead extraction opening provides a passage from the gap to an exterior space of the cartridge and is configured to removably receive the bead manipulation magnet for enabling an extraction of the magnetic beads from the microfluidic droplet by a removal of the bead manipulation magnet.

Cartridge, electrowetting sample processing system and bead manipulation method

A pipette tip extension attachable to a pipette tip having a proximal end, a distal end, and an exterior wall extending between the proximal end and the distal end is disclosed. The exterior wall has an outer side and an inner side and forms at the proximal end a reception aperture for inserting a pipette tip, and at the distal end a dispense aperture. The pipette tip extension further has an inner cavity, a distance element connected to the inner side of the exterior wall, and a constriction element.

Pipette tip extension for treating a sample with a liquid attachable to a pipette tip

A cartridge, in particular a disposable cartridge, for use in an electrowetting sample processing system. The cartridge has a liquid input port for introducing an input liquid into an internal gap of the cartridge, the input liquid providing for at least one droplet, directly or via a liquid separation process within the internal gap, and the internal gap having at least one hydrophobic surface, at least one processing zone for processing samples located in the processing zone, and a delivery zone for delivering the at least one droplet from the liquid input port to the at least one processing zone. The delivery zone is configured to provide a repeating pattern of interacting electrowetting force for simultaneously transporting the at least one droplet within the delivery zone.

Cartridge and electrowetting sample processing system with delivery zone

A cartridge for use in an electrowetting sample processing system, the cartridge having at least one inlet port for introducing an input liquid in an internal gap of the cartridge, wherein the gap has at least one hydrophobic surface and is configured to provide an electrowetting induced movement of a microfluidic droplet of input liquid, wherein the input liquid has a carrier liquid and a processing liquid and the gap has a capture zone that is configured to capture at least a part of the processing liquid as a microfluidic droplet by use of electrowetting force and the gap further has a transfer zone that is configured to provide a passage for the carrier liquid next to the microfluidic droplet, while processing liquid is captured in the capture zone.

Cartridge, electrowetting sample processing system and droplet formation

Methods and systems are disclosed for controlling a particle analyzer based at least in part on a generated entrainment factor for a sample. The features include flowing a sample with a series of particles through the particle analyzer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the particle analyzer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.

Sample behavior analyzer control

Methods and systems are disclosed for generating an entrainment factor in a flow cytometry sample. The methods comprise flowing a sample with a series of particles through the flow cytometer, detecting events and calculating an expected frequency of those events based on a distribution, such as a Poisson distribution, and measuring an observed frequency of particle events. An entrainment factor may be generated from a ratio of observed event frequency to expected event frequency. Further adjustment to the flow cytometer maybe performed based on the indicated entrainment factor such as adjusted sorting bias.

Methods and systems for assessing sample behavior in a flow cytometer

The invention provides a flow system and method for reliable multiparameter data acquisition and particle sorting. In accordance with the invention, a flow system assesses changes in the pattern of data collected in successive time intervals and actuates one or more corrective actions whenever the changes exceed predetermined limits. The present invention overcomes problems associated with collecting data and sorting and enumerating particles in flow cytometry systems that operate for prolonged periods or that must accommodate samples that vary widely in quality.

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San Jose, CA, United States of America

Sujata Iyer