Realnetworks Gmbh

Nautilus Subsidiary, Inc.

Nautilus Biotechnology, Inc.

Isilon Systems, Inc.

Intel Corporation

Emc Corporation

Sujal M Patel

Parag Mallick

Jeffrey M Ayars

Bradley D Hefta-Gaub

Rahul Agarwal

Dale R Stammen

Philip Rosedale

Paul A Mikesell

Darren P Schack

Jarrett D Egertson

Alan Francis Lippman

Bryan D Vergato

Gary S Greenbaum

Aaron Passey

David Stern

Peter S Haight

Dmitriy Gremyachinskiy

Vadim Lobanov

Ryan Kempston Seghers

Rachel Galimidi

Pierre F Indermuhle

Elliott Sorelle

The present disclosure provides systems and methods for detecting components of an array of biological, chemical, or physical entities. In an aspect, the present disclosure provides a method for detecting an array of biological, chemical, or physical entities, comprising: (a) using one or more light sensing devices, acquiring pixel information from sites in an array, wherein the sites comprise biological, chemical, or physical entities that produce light; (b) processing the pixel information to identify a set of regions of interest (ROIs) corresponding to the sites in the array that produce the light; (c) classifying the pixel information for the ROIs into a categorical classification from among a plurality of distinct categorical classifications, thereby producing a plurality of pixel classifications; and (d) identifying one or more components of the array of biological, chemical, or physical entities based at least in part on the plurality of pixel classifications.

Methods and systems for computational decoding of biological, chemical, and physical entities

The present disclosure provides methods and systems for performing single-molecule detection using fabricated integrated on-chip devices. Provided herein is a composition, comprising: (a) a substrate; (b) a structured nucleic acid particle coupled to the substrate, wherein a biological entity is coupled to the structured nucleic acid particle; and (c) a fluorescent detection agent, wherein the fluorescent detection agent comprises an affinity reagent attached to another structured nucleic acid particle, wherein the affinity reagent is bound to the biological entity.

Methods and systems for integrated on-chip single-molecule detection

Methods and systems are provided for accurate and efficient identification and quantification of proteins. In an aspect, disclosed herein is a method for identifying a protein in a sample of unknown proteins, comprising receiving information of a plurality of empirical measurements performed on the unknown proteins; comparing the information of empirical measurements against a database comprising a plurality of protein sequences, each protein sequence corresponding to a candidate protein among a plurality of candidate proteins; and for each of one or more of the plurality of candidate proteins, generating a probability that the candidate protein generates the information of empirical measurements, a probability that the plurality of empirical measurements is not observed given that the candidate protein is present in the sample, or a probability that the candidate protein is present in the sample; based on the comparison of the information of empirical measurements against the database.

Decoding approaches for protein identification

Methods for identifying a protein in a sample of unknown proteins

The methods described herein provide a means of producing an array of spatially separated proteins. The method relies on covalently attaching each protein of the plurality of proteins to a structured nucleic acid particle (SNAP), and attaching the SNAPs to a solid support.

Methods of generating nanoarrays and microarrays

A system and a method determine network conditions. The system includes a server computer and a client computer. The server computer is configured to transmit data packets comprising a data object from the server computer to the client computer. The client computer includes a transmission latency detector and a transmission bandwidth detector. The transmission latency detector uses transmission time and receipt time values to determine the changes in time it takes selected portions of the data object to be transmitted from the server computer to the client computer. The transmission bandwidth detector uses identified back-to-back data packets to determine the transmission bandwidth between the server computer and the client computer.

System and method for determining network conditions

A system and method provide random access to a multimedia object over a network. One embodiment of the invention includes a streaming media server that is connected to a client computer over a network. The streaming media server includes at least one multimedia object that is adapted for transmission ('streaming') across the network. A pre-roll calculation program determines a pre-roll for each segment of the multimedia object and generates a modified multimedia object which includes a pre-roll for each of its segments, a pre-roll identifying a portion of the multimedia object to be transmitted by the streaming media server and received by the client computer prior to playback of the multimedia object at a selected segment. The pre-roll allows the client computer to present, starting at a selected segment, the multimedia object in its entirety without interruption. The pre-roll calculation program further facilitates the design of the multimedia object to minimize delays associated with random accesses to segments of the multimedia object.

System and method for providing random access to a multimedia object over a network

A distributed file system enables the intelligent storing and retrieval of files among a plurality of storage units. The distributed file system utilizes a metadata data structure to track and manage detailed information about each file, including, for example, the device and block locations of the file's data blocks, to permit different levels of replication and/or redundancy. The distributed file system includes a switch to receive a read request for a file and to send the read request to one of the storage units. Each of the plurality of storage units is operable to monitor access to the file, such that a block cache module is operable to perform a scalable number of packet read aheads, wherein the scalable number is based on at least a current read rate and a current latency of a link to access the at least one file.

Distributed file system for intelligently managing the storing and retrieval of data

The intelligent distributed file system enables the storing of file data among a plurality of smart storage units which are accessed as a single file system. The intelligent distributed file system utilizes a metadata data structure to track and manage detailed information about each file, including, for example, the device and block locations of the file's data blocks, to permit different levels of replication and/or redundancy within a single file system, to facilitate the change of redundancy parameters, to provide high-level protection for metadata, to replicate and move data in real-time, and so forth.

Systems and methods for providing a distributed file system utilizing metadata to track information about data stored throughout the system

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Seattle, WA, United States of America

Sujal M Patel