Helicos Biosciences Corporation

Molecular Assemblies, Inc.

Modernatx, Inc.

Moderna Therapeutics, Inc.

Fluidigm Corporation

Suhaib Siddiqi

J William Efcavitch

Jason P Schrum

Atanu Roy

Kenechi Ejebe

Subramanian Marappan

Judith Mitchell

Philip Richard Buzby

Edyta Krzymanska-Olejnik

Jayson Linn Bowers

Xiaopeng Bai

Antonin De Fougerolles

Stephane Bancel

Paul Hatala

Mirna Jarosz

Hernan Orgueira

Mirna Jaroza

Edyta Olejnik

William J Efcavitch

The present disclosure provides modified nucleosides, nucleotides, and nucleic acids, and methods of using thereof.

Modified nucleosides, nucleotides, and nucleic acids, and uses thereof

The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template. Because the nucleotide analogs have an unmodified 3' OH, i.e., as found in 'natural' deoxyribose and ribose molecules, the analogs result in natural polynucleotides suitable for incorporation into biological systems.

Methods and apparatus for synthesizing nucleic acids

The present disclosure provides methods of increasing the level of a polypeptide of interest in a mammalian subject by administering a polynucleotide having one or more chemical modifications and a Protein:Cytokine ratio of greater than 100.

The invention generally relates to nucleotide analogs and methods of their use in sequencing-by-synthesis reactions. In certain embodiments, the invention provides a nucleotide analog including a detectable label attached to a nitrogenous base portion of a nucleotide analog by a cleavable linker, in which contact of the analog with at least one activating agent results in cleavage of the label and elimination of the linker, thereby producing a natural nucleotide, a 9-deaza-G, 9-deaza-A, or &#x3c8;-uridine.

Nucleotide analogs

The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids.

The invention provides a family of tethered nucleotide analogs useful in sequencing nucleic acids containing a homopolymer region comprising, for example, two or more base repeats, and to sequencing methods using such tethered nucleotide analogs.

Methods and compositions for sequencing a nucleic acid

The disclosure provides nucleotide analogs and methods of their use. Analogs of the invention comprise a reporter molecule (label) attached via the N4, N6, O4, or O6 position of the nitrogenous base portion of the analog. In a preferred embodiment, nucleotide analogs of the invention comprise a label attached to the nitrogenous base portion of the analog via a cleavable linker at the N4, O4, N6 or O6 position.

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Burlington, MA, United States of America

Suhaib Siddiqi