University System of Maryland

Sufi Rizwan Ahmed

Peter Kofinas

Steven Bullock

Timothy Alan Barbari

Compositions and methods are taught for directing the orientation of an immobilized capture biomolecule on a hydrophobic membrane. The method comprises layering at least one tie layer on a hydrophobic membrane, adding an amine functional layer on top of at least one tie layer; and attaching an alignment biomolecule to the amine functional layer. The alignment biomolecule has the ability to either capture a target biomolecule itself and thus be considered a capture biomolecule, or bind and orient the immobilized capture biomolecule so as to maximize the binding activity of the immobilized capture biomolecule. In one embodiment, a nickel-coordinated amine functional layer binds with a histidine-tagged alignment biomolecule. In another embodiment, an amine functional layer reacts, via tyrosinase catalysis, with a tyrosine residue in an alignment biomolecule.

Affinity membrane for capture of a target biomolecule and formation thereof by site-directed immobilization of a capture biomolecule

A method of room temperature synthesis of magnetic metal oxide nanoclusters within a diblock copolymer matrix includes the step of synthesizing, by ring opening metathesis polymerization technique, a diblock copolymer having a repeat unit ratio m/n, introducing, at room temperature, one or several metal containing precursors into the one block of the diblock copolymer, and processing the metal containing diblock copolymer by wet chemical technique to form nanoclusters of the metal(s) oxide within the diblock copolymer matrix. Specific reaction for synthesis of CoFeOand CoOnanoclusters within diblock copolymers, such as [NOR]/[NORCOOH]and [NOR]/[CO(bTAN)], respectively is used in the method of the present invention.

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Hillsboro, OR, United States of America

Sufi Rizwan Ahmed