Eli Lilly and Company

Suellen M Samson

Paul Luther Skatrud

Thomas D Ingolia

Stephen W Queener

Otis W Godfrey

The present invention provides DNA compounds that encode the expandase/hydroxylase enzyme of,. The compounds can be used to construct recombinant DNA expression vectors for a variety of host cells, including,Penicillium, and Cephalosporium.

Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase

The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.

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Indianapolis, IN, United States of America

Suellen M Samson