Life Technologies Corporation

Oxoid Limited

Sueh-Ning Liew

Pius Brzoska

Manohar R Furtado

Craig Cummings

Olga Petrauskene

Robert Tebbs

Jen-Kuei Liu

Michael Brewer

Manohar Furtado

Catherine O'CONNELL

Michael Schumaker

Sharon Matheny

The present teachings generally relate to methods and kits incorporating a discriminating positive control for determining whether a particular microorganism or group of microorganisms are present in a sample, for example but not limited to a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample. According to certain methods, at least part of a starting material, for example but not limited to, a food, environmental, agricultural, biopharmaceutical, pharmaceutical, or water sample can be combined with a culture medium and incubated under conditions suitable for microbial growth followed by extracting microorganism and added control nucleic acids for analysis. The extracted nucleic acids are amplified and the amplified nucleic acids are detected, directly or indirectly, and the fidelity of the methods and the presence or absence of the corresponding microorganism is determined because the discriminating positive control provides both confirmatory results for the methods used but eliminates false positive results.

Discriminatory positive/extraction control DNA

Growing community of inventors

Union City, CA, United States of America

Sueh-Ning Liew