Martek Biosciences Corporation

Martek Limited

Sue E Reeb

David J Kyle

Valerie J Sicotte

A biomass is provided which consists essentially of cells of dinoflagellates of the class Dinophyceae which have been cultivated to produce high cell mass and high DHA content. Biomass consisting of cells of Crypthecodinium cohnii is preferred. The cells are cultivated in an aerobic fermentor to achieve a cell density of a least 10 grams biomass per liter of nutrient solution by maintaining in the fermentor a dissolved oxygen level of at least about 10% of air saturation, and by inducing a stationary phase with a limiting nitrogen source in the nutrient solution. Under these conditions a single cell oil which contains at least 20% docosahexaenoic acid (DHA) in the triglyceride form at a concentration of at least about 1.5 grams per liter of nutrient solution is produced in the biomass. The biomass is suitable as an aquaculture feed.

Dinoflagellate biomass, methods for its production, and compositions

A pharmaceutical composition and a dietary supplement comprising a single cell oil containing at least 20% docosahexaenoic acid (DHA) in triglyceride form are presented. The single cell oil is extracted from biomass produced by cultivating heterotrophic microalgae of the class Dinophyceae in an aerobic fermentor with a nutrient solution having a limited nitrogen source.

Pharmaceutical composition and dietary supplement containing

This invention relates to a process for producing a single cell edible oil containing DHA, to the oil itself and to uses for the oil. Marine microorganisms are cultivated in fermentors and induced to produce the single cell oil which subsequently is recovered by extraction with solvents.

Growing community of inventors

Baltimore, MD, United States of America

Sue E Reeb