Council of Scientific and Industrial Research

Other

Department of Biotechnology

Sudeep Tandon

Atul Prakash Kahol

Madan Mohan Gupta

Sushil Kumar

Suman Preet Singh Khanuja

Rajendra Singh Bhakuni

Ram Kishor Verma

Mahendra Pandurang Darokar

Anil Kumar Singh

Dharam Chand Jain

Ravi Prakash Bansal

Anil Kumar Gupta

Ajit Kumar Shasany

Alok Kalra

Ram Prakash Sharma

Raj Kishori Lal

Asish Kumar Bhattacharya

Shiv Kumar Gupta

Mansoor Alam

Tarun Kumar Singh

Anirban Pal

Arun Kumar Kukreja

Guru Das Bagchi

Amit Tewari

Shilpi Paul

Suman Preet Khanuja

Govind Ram

Anuraddha Kumar

Neerja Pant

Guru Das Bagachi

Kiran Lata Singh

Deeptanjali Sahoo

Mohammed Shafique Siddique

Rajendra Prasad Patel

Tanpreet Kaur

The invention provides a simple method for isolation of calliterpenone (16&#x3b1;,17 dihydroxy-3-oxo phyllocladane), a phyllocladane diterpenoid with the plant growth regulating properties, from plant genus

Process for the isolation of calliterpenone

The present invention is related to the development of a novel, distinct high herb and artemisinin yielding genotype ofobtained through systematic marker assisted breeding followed by selection of uniform population in a methodical way wherein the genotype is distinct, uniform and stably maintainable by continuous rouging of off types in the population using DNA marker at early seedling stage from nursery itself and suitable for commercial cultivation.

High artemisinin yielding artemisia plant named 'CIM-arogya'

The present invention provides a novel process for the preparation of bacosides enriched fraction in a non-hygroscopic form the extract of , the said process comprising the steps of drying freshly harvested herb in a hot air oven at 37-42&deg; C., powdering and sieving the dried herb to obtain powder of 30-40 mesh size, defatting the powdered herb with hexane in a modified soxhlet extractor, extracting the defatted powdered herb with acetone, again extracting the same herb with methanol to obtain an extract containing bacosides, concentrating the extract to one twentieth of its original volume under reduced pressure, gradually adding the concentrated extract to acetone for precipitating the bacosides, filtering the bacosides in a Nutsche type vacuum filter, dissolving the crude bacoside mass into 2-10 parts water, extracting the bacoside solution with n-butanol to selectively transfer the bacosides to the solvent phase, separating and concentrating the solvent phase under vacuum to obtain semi-dry mass, dissolving the semi dried mass into 2-10 parts water, adding and stirring 1-5% of &bgr;-cyclodextrin to stabilize the bacosides, spray drying the stabilized bacoside solution by maintaining hot air temperature at 90-110&deg; C., to obtain a stable free flowing fraction of rich in bacosides.

Process for the preparation of a extract rich in bacosides from the herb Bacopa monniera

The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.

Process for the isolation of compound scopoletin useful as nitric oxide synthesis inhibitor

The invention relates to a novel process for the isolation of a hepatoprotective agent Silymarin from the seeds of the plant,comprising (i) Precooling the seeds to (&minus;) 20&deg; C. for 24 hours in a deep freezer/cold room. (ii) Powdering the cooled seeds in a hammer mill, fitted with about 40 mesh discharge screen. (iii) Defatting the seeds by extracting with hexane in a soxhlet type extractor to remove the total quantity of fatty oil without using a scrain. (iv) Extracting the defatted seeds with acetonitrile at 20-30&deg; C. to extract silymarin fraction. (v) Concentration of the sensitive silymarin fraction under vacuum in a agitated wiped thin film evaporator (vi) stirring the silymarin such dry powder with cold dichloromethane at 5&deg; C. followed by filtration and drying with a slow purge of nitrogen gas. (vii) Further purification of silymarin by suspending in 5 times its weight of acetonitrile and precipitating by 8-12 times its weight of water at 20&deg; C. (viii) Filtering the precipitated silymarin in a closed vacuum filter and having 1-2 &mgr;m screen washing the cake three times with distilled water (ix) Drying of Silymarin cake in vacuum oven to obtain substantially pure silymarin.

Process for isolation of hepatoprotective agent silymarin from the seeds of the plant Silybum marianum

This invention relates to a novel process for the dual production of essential oil and artemisinin from the plant Artemisia annua, said process comprising extracting the plant with hexane, partitioning the hexane extract between hexane and acetonitrile, hydrodistillation of hexane residue and Marc to yield essential oil, further fractionation of acetonitrile solvent between hexane-benzene mixture to remove artemisinic acid, chromatographing of the acetonitrile phase to produce substantially pure artemisinin, the artemisinic acid is reduced to obtain dihydro artemisinic acid which is then oxidised in a single step to form artemisinin.

Process for the simultaneous production of artemisnin and essential oil

Growing community of inventors

Lucknow, India

Sudeep Tandon