Duke University

Sudarshan Rajagopal

Bastiaan Driehuys

Marc G Caron

Thomas Pack

Ziyi Wang

Jeffrey Smith

Elianna Bier

Methods and systems withXe dynamic spectroscopy with a fitting function that includes one or more non-Lorentzians, optionally with a barrier Voigt, and signal processing for identifying cardiogenic oscillations for evaluating disease states, use in drug discovery or monitoring disease status.

Dynamic 129Xe gas exchange spectroscopy

Provided are methods for detecting protein interactions in a sample, the methods comprising: (a) detecting two or more polypeptides that when associated emit a first detectable signal in a first light emission spectrum; (b) contacting the two or more polypeptides with a third polypeptide conjugated to a dipole acceptor moiety that has a second light emission spectrum when excited within a light excitation spectrum, wherein the light excitation spectrum overlaps with the first light emission spectrum; and (c) detecting a second detectable signal emitted in the second light emission spectrum by the dipole acceptor moiety. Also provided are bioluminescent complexes comprising: (a) a first polypeptide conjugated to a dipole acceptor moiety, wherein the emits a first detectable signal in a first light emission spectrum.

Complex BRET technique for measuring biological interactions

Growing community of inventors

Durham, NC, United States of America

Sudarshan Rajagopal