Immunex Corporation

Other

Subhashini Srinivasan

Melanie K Spriggs

Richard Jeffrey Armitage

William Christian Fanslow, Iii

Marylou G Gibson

Arvia Eleanor Morris

Jeffrey T McGrew

Padmanaban Sudarsanam

There is disclosed a polypeptide (CD40-L) and DNA sequences, vectors and transformed host cells useful in providing CD40-L polypeptides. More particularly, this invention provides isolated human and murine CD40-L polypeptides that bind to the extracellular binding region of a CD40 receptor.

DNA encoding CD40 ligand, a cytokine that binds CD40

There is disclosed a polypeptide (CD40-L) and DNA sequences, vectors and transformed host cells useful in providing CD40-L polypeptides. More particularly, this invention provides isolated human and murine CD40-L polypeptides that bind to the extracellular binding region of a CD40 receptor. Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ligand polypeptide that stimulates B cell proliferation.

Use of CD40 ligand, a cytokine that binds CD40, to stimulate hybridoma cells

Method of augmenting a vaccine response by administering CD40 ligand

There is disclosed a polypeptide (CD40-L) and DNA sequences, vectors and transformed host cells useful in providing CD40-L polypeptides. More particularly, this invention provides isolated human and murine CD40-L polypeptides that bind to the extracellular binding region of a CD40 receptor. Also disclosed are methods of inhibiting undesirable immune responses, preventing T cell interaction with B cells by blocking CD40L binding to CD40 sites on B cells and other target cells.

Methods of inhibiting CD40L binding to CD40 with soluble monomeric CD40L

Recombinant soluble CD40 ligand polypeptide and pharmaceutical

A method in a computer system for modeling a three-dimensional structure of a model protein is provided. In one embodiment, the modeling is based upon a three-dimensional structure of a template protein and an amino acid sequence alignment of the model protein and the template protein. For each amino acid in the model protein, when the template protein has an amino acid aligned with the amino acid of the model protein, the position of the backbone atom of the amino acid of the model protein is established based on the position of a topologically equivalent backbone atom in the aligned amino acid of the template protein. In another embodiment, the modeling of a variable region of the model protein is based on a collection of .psi. and .phi. angle values for amino acid pairs in a family of proteins. In a further embodiment, these .psi. and .phi. angle values are classified according to a tetramer of adjacent amino acids and filtered based on a most probable conformation of portions of the variable region of the model protein.

Method and system for protein modeling

There is disclosed a method of preparing a soluble mammalian protein by culturing a host cell transformed or transfected with an expression vector encoding a fusion protein comprising a zipper domain and a heterologous mammalian protein.

Methods of preparing soluble, oligomeric proteins

A method in a computer system for modeling a three-dimensional structure of a model protein is provided. In a preferred embodiment, the modeling is based upon a three-dimensional structure of a template protein and an amino acid sequence alignment of the model protein and the template protein. The proteins comprise a plurality of amino acids having backbone atoms and side chain atoms. For each amino acid in the model protein, when the template protein has an amino acid aligned with the amino acid of the model protein, the position of each backbone atom of the amino acid of the model protein is established based on the position of a topologically equivalent backbone atom in the aligned amino acid of the template protein. The inter-atomic distance constraints for each pair of atoms with an established position is generated. Finally, the position of each atom in the model protein is set so that the inter-atomic distances are in accordance with the constraints.

Growing community of inventors

Kirkland, WA, United States of America

Subhashini Srinivasan