The United States Government As Represented by the Department of Veterans Affairs

Loma Linda University

Other

Boehringer Mannheim Gmbh

Subburaman Mohan

David J Baylink

Kin-Hing William Lau

Shin-Tai Chen

Susan L Hall

The present disclosure provides compositions and methods for increasing bone growth and/or enhancing wound healing, for example, fracture repair. The disclosure provides recombinant nucleic acids useful for promoting bone growth. For example, the disclosure provides recombinant nucleic acids that encode a fibroblast growth factor-2 (FGF-2) analog. The disclosure also provides vectors and cells incorporating these nucleic acids, as well as FGF-2 analogs encode by them. The disclosure also provides a mouse system of bone marrow transplantation and methods for producing as well as methods for using the system. Methods for inducing division and/or inducing differentiation of a hematopoietic stem cell are also provided, as are methods for enhancing bone growth and/or wound repair (for example, fracture repair).

Compositions and methods for osteogenic gene therapy

Composition and methods for osteogenic gene therapy

The present invention provides methods for separating ligands from binding proteins. The methods include acidic separation and size separation. The methods of the present invention are particularly useful for separating insulin-like growth factors from insulin-like growth factor binding proteins.

Method for removal of binding proteins from ligands

The invention provides homogeneous compositions of a binding protein for insulin-like growth factors (IGFs) I and II, which binding protein inhibits the proliferative effects of IGF-I and IGF-II upon bone cells. The homogeneous protein of the invention is therefore known as inhibitory IGF binding protein, or In-IGF-BP. The invention also provides cloned nucleic acid sequences encoding In-IGF-BP, artificial expression systems for In-IGF-BP and host cells which produce useful quantities of In-IGF-BP. The invention further provides clinical diagnostic assays for IGFs, therapeutic treatments for IGF-responsive tumors, and means for modulating IGF activity upon bone cells.

Inhibitory binding protein for insulin-like growth factors

Growing community of inventors

Redlands, CA, United States of America

Subburaman Mohan