Panagene Inc.

Su Nam Kim

Goon Ho Joe

Jin Woo Kim

Sung Kee Kim

Heekyung Park

Se Ryun Kim

Jae Jin Choi

Sung-Kee Kim

In Seong Wo

Yongtae Kim

Ji Hye Yoon

Jun Ho Park

Yong Tae Kim

Chwang Siek Park

Disclosed is a probe mixture for real-time detection of target nucleic acids comprising at least one detection probe and at least one clamping probe for inhibiting amplification of wild type genes or unwanted genes, a kit using the same and a method for real-time detection of target nucleic acids using the mixture and the kit.

Method for the detection of multiple target nucleic acids using clamping probes and detection probes

Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.

Melting curve analysis using PNA probe, method and kit for analyzing nucleotide polymorphism using melting curve analysis

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Daejeon, South Korea

Su Nam Kim