Merck + Co., Inc.

The Medical College of Pennsylvania

Hahnemann Univ.

Su Jane Rutledge

Gideon A Rodan

Azriel Schmidt

Robert L Vogel

M Katharine Holloway

Eitan Friedman

A human protein tyrosine phosphatase (PTP) has been identified and its cDNA has been isolated. This PTP, denoted PTP-OB, has a receptor-like three dimensional structure and is present in osteoblasts. PTP-OB is involved in osteoblast differentiation, and modulators of PTP-OB activity in turn modulate osteoblast differentiation, osteoclast differentiation and osteoclast activity.

Method of identifying modulators of protein tyrosine phosphatase activity

Human steroid receptor

A novel human protein tyrosine phosphatase (PTP) has been identified and its cDNA has been isolated. This novel PTP, denoted PTP-OB, has a receptor-like three dimensional structure and is present in osteoblasts. PTP-OB is involved in osteoblast differentiation, and modulators of PTP-OB activity in turn modulate osteoblast differentiation, osteoclast differentiation and osteoclast activity.

Human protein tyrosine phosphatase OB protein

Wnt-x growth factor polypeptide, DNA encoding same, and Wnt-x antibody

The NER receptor is a novel member of the steroid hormone receptor superfamily and has been prepared by cDNA cloning from a human osteosarcoma SAOS-2/B10 cell library. The complete sequence of human NER complementary DNA (Seq. ID No. 1), expression systems including a COS stable expression system, the expressed protein (SEQ. ID No. 2) and an assay using the COS expression system are disclosed. The assay may be used to identify agents which activate transcription mediated by the NER receptor, and which are useful for potentiating the activity of a modulator of a G-protein coupled receptor wherein the G-protein coupled receptor is a member of the steroid hormone receptor superfamily.

Method for finding transcription activators of the NER steroid hormone

CDNA encoding a novel human protein tyrosine phosphatase

Growing community of inventors

East Greenville, PA, United States of America

Su Jane Rutledge