E.i. Dupont De Nemours and Company

Sustainable Technology Corporation

Stuart M Thomas

Vasantha Nagarajan

Qiong Cheng

Michael G Bramucci

Kristy N Kostichka

David Richard Corbin

Pierre E Rouviere

Mario W Chen

Katharine Janet Gibson

Carol M McCutchen

Luan Tao

Patricia Cheung

Patricia C Brzostowicz

Gregory Paul Shankwitz

Vidya Pai

Dominic A Dragotta

Ming Woei Lau

Kristy Nan-Shan Kostichka

Ryan Eric Warner

Bradley Curt Fox

Joseph Michael Selby

The present invention provides a process using zeolites for recovering isoflavones and removing undesired oligosaccharides from aqueous mixtures, such as soy whey and other plant processing waste products. The aqueous mixture is treated with a large pore hydrophobic zeolite, such as zeolite Beta, to remove isoflavones, followed by treatment with an ultrastabilized, hydrophobic zeolite Y, such as CBV-901 or HiSiv&#x2122; 4000, to remove the undesired oligosaccharides raffinose and stachyose. The recovered isoflavones and digestible sugars, glucose, sucrose, and fructose, are useful in food products.

Recovery of isoflavones and removal of oligosaccharides from aqueous mixtures using zeolites

The present invention relates to a process for selectively removing undesired oligosaccharides from aqueous mixtures such as plant processing waste products, including soy whey and other vegetable wheys, using an ultrastabilized large pore, hydrophobic zeolite Y. The resulting solution, which contains isoflavones and digestible sugars such as glucose, fructose and sucrose, can serve as the basis for a nutritious new product.

Selective removal of oligosaccharides from aqueous mixtures using zeolites

Genes have been isolated from a variety of bacteria encoding Baeyer-Villiger monooxygenase activity. The genes and their products are useful for the conversion of ketones to the corresponding esters. A series of motifs, common to all genes, has been identified as diagnostic for genes encoding proteins of this activity.

Genes encoding Baeyer-Villiger monooxygenases

Genes have been isolated fromAN12 strain encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from astrain. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

Genes involved in isoprenoid compound production

A method has been developed to maintain the health of an activated sludge environment in a wastewater process comprising monitoring the levels of polyhydroxyalkanoates (PHA) produced as an indicator of bioreactor health and regulating the concentration of feed nutrients and nitrate to maintain bioreactor health. In general, levels of PHA in excess of about 15% to about 20% dry weight of the biomass is an indication that the biocatalytic efficiency of the wastewater treatment process is impaired.

Method for the use of polyhydroxyalkanoate levels to maintain bioreactor health

A 10 kb gene cluster has been isolated from SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.

Genes involved in cyclododecanone degradation pathway

A gene cluster has been isolated from an Acinetobacter sp. that encodes the enzymes expected to convert cyclohexanol to adipic acid. The entire gene cluster has been cloned and all open reading frames have been sequenced. Cosmid clones have been identified containing the gene cluster. Demonstration of conversion of cyclohexanol to adipic acid has been made with the recombinant host strain containing the cosmids.

Biological method for the production of adipic acid and intermediates

A method has been developed to monitor the health of an activated sludge environment in a wastewater process comprising monitoring the levels of polyhydroxyalkanoates (PHA) produced and correlating those levels with various selected sample parameters. In general, levels of PHA in excess of about 15% to about 20% dry weight of the biomass is an indication that the biocatalytic efficiency of the wastewater treatment process is impaired.

Polyhydroxyalkanoate levels as an indicator of bioreactor health

This invention relates to a biocatalytic process to produce terephthalic acid and isophthalic acid from p-xylene and m-xylene, respectively. Terephthalic acid has been prepared by oxidizing p-xylene with bacteria belonging to the genus Burkholderia. Conversion of p-xylene into terephthalic acid is accomplished by a single bacterial strain that produces all of the requisite enzymes. In addition, this invention relates to the preparation of isophthalic acid from a mixture of m- and p-xylene.

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Wilmington, DE, United States of America

Stuart M Thomas