Arizona State University

Molecular Imaging Corporation

Arizona Board of Regents, a Body Corporate

Other

Recognition Analytix, Inc.

Agilent Technologies, Inc.

Aeromax Technologies, Inc.

USA as Represented by Secretary of the Navy

Columbia University

Molecular Imaging Corporation Arizona Board of Regents

Stuart Lindsay

Peiming Zhang

Tianwei Jing

Yanan Zhao

Jin He

Pei Pang

Wenhai Han

Kevin Reinhart

Shuo Huang

Brett Gyarfas

Shuai Chang

Alexander A Gall

Allan J Wachter

James H Wagner

Subhadip Senapati

Sudipta Biswas

Sovan Biswas

Suman Sen

Yuri L Lyubchenko

Nongjian Tao

Joshua LaBaer

Hao Yan

Colin P Nuckolls

Karen Anderson

John C Chaput

Steven K Harbaugh

John Devens Gust, Jr

Xu Wang

Jinyao Tang

Saikat Manna

Peter Hinterdorfer

Jeremy Nelson

Manfred Philipp

Bintian Zhang

Jong One Im

Hanqing Deng

Sepideh Afsari Mamaghani

Mark Knappenberger

Jacob L Swett

Hongda Wang

Xiaodong Cui

The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure relates to methods for sequencing a polynucleotide using a bioelectronic device that obtains a bioelectronic signature (e.g., current amplitude levels) of polymerase activity based on current fluctuations as complementary nucleotidepolyphosphate monomers (e.g., having distinct charges) are incorporated into the template polynucleotide.

Methods for sequencing biopolymers

DEVICE, SYSTEM AND METHOD FOR DIRECT ELECTRICAL MEASUREMENT OF ENZYME ACTIVITY

A universal connection system for assembling and electrically connecting proteins to make bioelectronic detectors and logic circuits, exploiting the electronic properties of ligand-receptor interactions and the quasi metallic properties of protein interiors.

Bioelectronic circuits, systems and methods for preparing and using them

A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of L1 and L2.

System and method for single molecule detection

The present disclosure provides devices, systems, and methods related to single molecule detection. In particular, the present disclosure provides devices and methods for sequence-specific detection of a nucleic acid target using current fluctuations as a readout for protein binding to the nucleic acid target. As described herein, certain aspects of the bioelectronic devices and method can be used to detect and identify any nucleic acid target for the purpose of diagnosis and/or treatment.

Single-molecule electronic sequence detector and methods of use

The present disclosure provides devices, systems, and methods related to bioelectronic devices. In particular, the present disclosure provides bioelectronic devices, and methods of making bioelectronic devices, comprising adjustable adaptor polypeptides with repeatable motifs and enhanced conductive properties. The bioelectronic devices and methods of the present disclosure are useful for a variety of applications, including the direct measurement of protein activity (e.g., when sequencing a biopolymer).

Bioelectronic devices with programmable adaptors

The present disclosure relates to a sensing device and methods for detecting, and quantifying the amount of, a target protein in a sample.

Method for electronic detection and quantification of antibodies

A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length LI and is configured to selectively bind to a target molecule having an effective length L. The size of the gap is configured to be greater than L, but less than or equal to the sum of LI and L

The present disclosure provides devices, systems, and methods related to sequencing a biopolymer. In particular, the present disclosure provides methods of obtaining a bioelectronic signature based on current fluctuations that correspond to the activity of an enzyme-of-interest. As described herein, certain aspects of the bioelectronic signature can be used to determine the sequence of a biopolymer.

The present disclosure relates to a device, system and method for sensing functional motions of a single protein molecule via direct attachment of one or more electrodes to the molecule. The present disclosure also relates to an array, a system comprising an array and method for sequencing a biopolymer using an array.

Device, system and method for direct electrical measurement of enzyme activity

The present disclosure provides a method for identifying and quantifying sulfated glycosaminoglycans, including for example heparin, by passing a sample through nanopores. The glycosaminoglycans sample is measured in microliter quantities, at nanomolar concentrations with detection of impurities below 0.5%, and a dynamic range over five decades of magnitude with a trained machine learning algorithm.

Solid state nanopores aided by machine learning for identification and quantification of heparins and glycosaminoglycans

Embodiments of the disclosure are directed to a device for molecule sensing. In some embodiments, the device includes a first electrode separated from a second electrode by a dielectric layer. The first electrode comprises a large area electrode and the second electrode comprises a small area electrode. At least one opening (e.g., trench) cut or otherwise created into the dielectric layer exposes a tunnel junction therebetween whereby target molecules in solution can bind across the tunnel junction.

Systems and devices for molecule sensing and method of manufacturing thereof

The present disclosure provides apparatus and methods for determining the sequence of a nucleic acid. The apparatus comprises electrodes that form a tunnel gap through which the nucleic acid can pass. The electrodes comprise a reagent that is capable of selectively interacting with a nucleobase of the nucleic acid sequence. When the reagent interacts with a nucleobase, a detectable signal is produced and used to identify the nucleobase of the nucleic acid. Advantageously, the apparatus of this disclosure is specific to identifying nucleic acids.

Non-hydrogen-bonding universal reader for DNA sequencing

The present disclosure provides systems, devices, and methods that relate to a molecular recognition device configured to at least one of sense, identify and sequence at least one portion of a target molecule, the device comprising (a) a partition having a first side and a second side; (b) at least one constriction having a first end open to the first side and a second end open to the second side; (c) at least one pair of first and second sensing electrodes arranged within the constriction between the first side and the second side of the partition; and (d) a layer of a porous material at least one of arranged and formed on the first side of the partition.

Porous material functionalized nanopore for molecular sensing apparatus

Growing community of inventors

Phoenix, AZ, United States of America

Stuart Lindsay