Thomas Jefferson University

University of Louisville Research Foundation, Inc

Tissue Genesis Incorporated

The University of Arizona

Becton Dickinson and Company Limited

Other

Arizona Board of Regents, a Body Corporate

Surmodics, Inc.

Tissue Genesis International LLC

Becton

Stuart K Williams

Bruce E Jarrell

James Beatty Hoying

Paul G Alchas

Joseph A DiPisa

Erik Vossman

Paul Kosnik

Jonathan B Gabel

Christian Leon Haller

Frank A Augello

Charles P Luddy

Thomas F Cannon

James B Hoying

Deborah G Rose

Jeremy S Touroo

Gregory D Ariff

Jennifer L Case

Craig A Mauch

Benjamin R Shepherd

Paul J Mulhauser

Christopher John Brooks

Amanda J LeBlanc

Tony A Cutshall

Wes Prais

Robert Glenn Dennis

Alfred Wesley Prais

David L Clapper

Robert W Snyder

R Anthony Carabasi

Alison Stopeck

Pauline K Park

Thomas L Carter

Anthony Yang

Rolf Wolters

Josh Nelson

Carlos C Chang

Balamurugan Appakalai

Piyani S Gandhi

Joseph S Zakhari

Brian C Gettler

Laxminarayanan Krishnan

Christopher T England

Hyun Joon Paek

Eugene D Boland

Methods of making a spheroid are provided whereby a suspension is first produced including one or more biologically-relevant materials dispersed within a biocompatible medium. A droplet of the suspension is then bioprinted into a salt solution by bringing the droplet into contact with a surface of the salt solution in a controlled manner to reproducibly yield a spheroid containing a desired size and a desired amount of biologically-relevant materials.

Methods of making spheroids including biologically-relevant materials

Systems and methods herein are directed towards the separation of biologic material to obtain a target cell volume and/or cell concentration for harvesting. The target volume and/or concentration of cells may be obtained through a single cycle via three chambers, or by repeated cycles through one or more chambers to dilute the digestive enzymes used in the process and concentrate the harvestable cell volume to a predetermined target.

Cell separation apparatus

The present invention provides automated devices for use in supporting various cell therapies and tissue engineering methods. The present invention provides an automated cell separation apparatus capable of separating cells from a tissue sample for use in cell therapies and/or tissue engineering. The cell separation apparatus can be used in combination with complementary devices such as cell collection device and/or a sodding apparatus to support various therapies. The automated apparatus includes media and tissue dissociating chemical reservoirs, filters, a cell separator and a perfusion flow loop through a graft chamber which supports a graft substrate or other endovascular device. The present invention further provides methods for using the tissue grafts and cell samples prepared by the devices described herein in a multitude of therapies including revascularization, regeneration and reconstruction of tissues and organs, as well as treatment and prevention of diseases.

Cell separation apparatus and methods of use

A tissue construct is provided that comprises a pancreas derived microvessel fragment and a pancreatic islet cell. The pancreas derived microvessel fragment and the pancreatic islet cell can be incorporated into a biocompatible medium. Tissue constructs can be comprised of other cells, such as stem cells, combined with the pancreas derived microvascular fragment. Methods for isolating microvessel fragments from a pancreas are also provided and include enzymatic digestion of pancreatic tissue and separation of microvessel fragments from endocrine and exocrine tissue. Methods for treating diabetes are further provided and include administration of the tissue constructs.

Tissue constructs including pancreas derived microvessel fragments and related methods

A method of making a spheroid is provided that includes the step of providing a suspension having one or more biologically-relevant materials dispersed within a biocompatible medium. An amount of a hydrophilic material is deposited on a defined area of a super-hydrophobic surface, and a droplet of the suspension is bioprinted onto the hydrophilic material positioned on the super-hydrophobic to thereby create the spheroid.

Spheroids including biologically-relevant materials and related methods

Three-dimensional tissue constructs are described, which may be created by isolating adipose-derived stromal vascular fraction (SVF) cells, plating the cells onto a polymer scaffold, and culturing the plated scaffold in a culture of DMEM with approximately 10% FBS.

Adipose stromal vascular fraction cell constructs

This application discloses methods for treating an established myocardial infarction, including treatment with an epicardial construct containing stromal vascular fraction (SVF) from adipose tissue which may be seeded onto a biocompatible substrate, which preserves microvascular function and LV contractile mechanisms.

Methods for treating an established myocardial infarction

The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. In some embodiments, the SVF cells can be on the surface or embedded within a three-dimensional matrix. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.

Methods and compositions to support transplanted tissue integration and innosculation with adipose stromal cells

This application discloses methods and materials for preparing functional microvascular beds in the laboratory. These prevascularized constructs can be used to vascularize engineered tissue constructs or to revascularize damaged or diseased tissues or organs following implantation. The prevascularized constructs may also deliver genetically engineered gene products to the bloodstream.

Prevascularized constructs for implantation to provide blood perfusion

A modular bioreactor is provided that includes an upper biochamber; a lower biochamber; and an intermediate biochamber that is positioned between the upper biochamber and the lower biochamber. Each biochamber of the bioreactor is in fluid communication with each other biochamber of the bioreactor and includes an interior wall, which defines a centrally-disposed cavity for each biochamber, and an inflow port and an outflow port that are in fluid communication with each centrally disposed cavity. Systems and methods for vascularizing a tissue construct are also provided.

Bioreactors, systems, and methods for vascularizing tissue constructs

The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. Another aspect provides a composition comprising an engineered corpus cavernosum tissue comprising SVF cells and corpus cavernosum cells, wherein the SVF cells can be mixed with or encasing the corpus cavernosum cells, and methods of uses thereof, for example in method for the treatment of impotence and erectile dysfunction and/or enhance or construct a penis. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.

Methods and compositions to support tissue integration and inosculation of transplanted tissue and transplanted engineered penile tissue with adipose stromal cells

Growing community of inventors

Tucson, AZ, United States of America

Stuart K Williams