Wisconsin Alumni Research Foundation

Other

Mirus Corporation

The United States of America, As Represented by the Secretary, Department of Health and Human Services

The UAB Research Foundation (The University of Alabama at Birmingham)

Knechtle, Stuart J.

Stuart J Knechtle

Jon Asher Wolff

Vladimir G Budker

David M Neville

Jue Wang

Majed M Hamawy

Huaizhong Hu

Shenglin Ma

Judith Thomas

David A Neville

A process for delivering a polynucleotide to a parenchymal cell in a mammal by injecting the polynucleotide into a blood vessel connected to the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell and functionally expressed to therapeutic levels.

Process of delivering naked DNA into a hepatocyte via bile duct

Disclosed herein are methods for monitoring the immunological status of transplant recipients. Fibroblast cells derived from transplanted organs are used in proliferation assays with lymphocytes taken from the recipient after transplantation. The comparison of assay results in the presence of, and in the absence of (or in the presence of reduced levels of) a cytokine growth factor such as interleukin-2, is used to determine whether the organ is being rejected. Also disclosed are kits for practicing these methods.

Assessing immunological state of transplant recipients

Disclosed is a method of inhibiting a rejection response by a primate to a transplanted organ. One exposes the primate to a mutant diphtheria toxin linked to anti-CD3 antibody so as to largely eliminate the host's peripheral blood T cell lymphocyte population. At the same time as, or after, the exposure step one administers to the primate's thymus gland donor lymphocytes. Transplantation of the organ follows. The primate is tolerized to the transplanted organ.

Thymus tolerance in primates

Growing community of inventors

Oregon, WI, United States of America

Stuart J Knechtle