Koninklijke Philips Corporation N.v.

Stuart Hamish Shand

Marinus Bastiaan Van Leeuwen

Bas Hulsken

Jelte Peter Vink

Martijn Wolters

The present invention relates to a system for generating a synthetic 2D image with an enhanced depth of field of a biological sample. It is described to acquire () with a microscope-scanner () first image data at a first lateral position of the biological sample and second image data at a second lateral position of the biological sample. The microscope-scanner is used to acquire () third image data at the first lateral position and fourth image data at the second lateral position, wherein the third image data is acquired at a depth that is different than that for the first image data and the fourth image data is acquired at a depth that is different than that for the second image data. First working image data is generated () for the first lateral position, the generation comprising processing the first image data and the third image data by a focus stacking algorithm. Second working image data is generated () for the second lateral position, the generation comprising processing the second image data and the fourth image data by the focus stacking algorithm. The first working image data and the second working image data are combined (), during acquisition of image data, to generate the synthetic 2D image with an enhanced depth of field of the biological sample.

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Stuart Hamish Shand