Eli Lilly and Company

Stuart A Kuhstoss

R Nagaraja Rao

Paul R Rosteck, Jr

Eugene T Seno

Brigitte E Schoner

Karen L Cox

Mark Alan Richardson

Mark A Richardson

Ramachandra N Rao

Ramamoorthy Belagaje

Bradley Stuart DeHoff

Stanley Gene Burgett

Kimberly L Sutton

A DNA molecule isolated from Streptomyces ambofaciens encodes the multi-functional proteins which direct the synthesis of the polyketide platenolide.

Platenolide synthase gene

A DNA molecule isolated from Streptomyces fradiae encodes the multi-functional proteins which direct the synthesis of the polyketide tylactone.

Polyketide synthase genes

The present invention provides a method for transforming an actinomycete with an integrating vector which has the advantages of high transformation rates into a broad host range, site-specific integration, and stable maintenance without antibiotic selection. Also provided are methods for the increased production of antibiotics and for the production of hybrid antibiotics.

Use of the site-specific integrating function of phage .phi.C31

A transcription terminator is disclosed comprising a DNA sequence capable of functioning as a transcription terminator in a microorganism of the order Actinomycetales. These transcription terminators are functional in both gram-positive and gram-negative microorganisms, especially Streptomyces ambofaciens. Additionally, a series of recombinant DNA cloning vectors suitable for isolating the disclosed transcription terminators are set forth.

Transcription terminator and related recombinant DNA vectors and

The invention relates to a method and cloning vehicle for the expression of a functional polypeptide in Streptomyces. A recombinant DNA cloning vehicle was genetically engineered to bring the expression of the neomycin phosphotransferase gene under the control of the Escherichia coli bacteriophage .lambda.p.sub.L promoter.

Method of using bacteriophage lambda p.sub.1 promoter to produce a

The present transcription terminators are DNA sequences characterized by their ability to terminate the transcription of DNA sequences. The present sequences are portable, have a variety of restriction endonuclease sites and are useful in controlling the expression in hosts of DNA sequences coding for prokaryotic, eukaryotic and viral proteins and polypeptides.

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Indianapolis, IN, United States of America

Stuart A Kuhstoss