Institut Pasteur

Etablissement Declare D'utilite Publique Dit. Institut Pasteur

Other

Agence Nationale De Valorisation De La Recherche (anvar)

Centre National De La Recherche Scientifique

Université Pierre Et Marie Curie

Institut Pasteur Paris

Etablissement Declare D'utilite Publique Diti Institut Pasteur

Stratis Avrameas

Jean-Luc Guesdon

Philippe Kourilsky

Therese Ternynck

Therese M Ternynck

Alexandre Avrameas

Gerard Buttin

Susan Michelson

Eric Selegny

Daniel Thomas

Georges Broun

Bruno Blondel

Brigitte Cami, Born Contamine

Therese Couderc

Donato Zipeto

Marie-Francoise Saron

Jean-Claude Mazie

Faridabano Nato

Brigitte Cami Contamine

Brigitte Cami Nee Contamine

Gérard Buttin

Thérèse Ternynck

Thérèse Couderc

Marie-Françoise Saron

The present invention provides novel polypeptides which can effectively penetrate into cells thereby transporting a substance of interest into the cells.

Vectors derived from antibodies for transferring substances into cells

Single-chain antibody fragments for transferring substances into cells

A product for coupling a biologically active principle with an immunovector, characterized in that the immunovector is capable of enabling the biologically active principle to be internalized into eukaryotic cells, and in that said immunovector has an affinity for the cell DNA to such an extent that it can transfer the biologically active principle into or to the immediate vicinity of the cell nuclei.

Immunovectors for the intracellular and intranuclear transport

Method for detecting the possible presence of a DNA fragment, notably of a gene, in the midst of a complex sample of nucleic acids. It comprises the hybridization of the sought fragment with a RNA probe, this being, prior or subsequent to the hybridization reaction, modified by an enzyme. Application to seeking of particular genes or DNA fragments in the midst of a biological sample.

Method of detecting and characterizing a nucleic acid or reactant for

Method of detecting and characterizing a nucleic acid or a sequence of

Detection methods and compositions of matter comprising an enzyme coupled to a nucleic acid are disclosed. A complex of the enzyme-nucleic acid hybridized to a nucleic acid to be detected is also disclosed. The nucleic acid is detected by measuring the activity of the enzyme.

This process involves the use of benzoquinone as cross-linking agent in large excess compared with the substance to be activated. The activation reaction is realized in a homogeneous liquid medium. Said process making it possible for example to couple antibodies to enzymes for determining or detecting said antibodies. The process can be used for the determination of antitetanic antibodies in human serum.

Products of coupling biological substances by covalent bonds

This method for immunological determination of amines in biological media consists of: (a) reacting a quinone (in particular, p-benzoquinone) with the amine (for example histamine) present in the test sample; (b) introducing into the medium a monoclonal antibody which specifically recognizes the amine-quinone reaction product formed; and (c) determining one of the forms of the monoclonal antibody present. When the quinone is used in the immobilized state, the monoclonal antibody present is determined in its complexed form with the amine-quinone reaction product (direct type method); when the quinone is used in the free state, the monoclonal antibody is determined in its free form (enzymoimmunometric method or competitive immunoenzymatic method).

Method for immunological determination of amines, monoclonal antibodies

A process for the detection and assay of a biological substance by erythroadsorption by immobilizing a substance having a binding affinity for the biological substance to be assayed; incubating the immobilized substance with a liquid medium containing the biological substance to be assayed, forming a fixed substance; incubating the fixed substance with a coupling product comprising a specific ligand and a second ligand which binds with erythrocytes; adding erythrocytes; and determining the amount of erythrocytes bound to the coupling product. The determination of the amount of bound erythrocytes can be made visually with the naked eye or determined by lysis of the erythrocytes bound to the coupling product and quantitatively measuring the released hemoglobin. The specific ligand can be an antibody or an antigen and the second ligand capable of coupling erythrocytes can, for example, be red blood cell antibodies.

Process for the detection and assay by erythroadsorption

Method for detecting and dosing by erythroadsorption a biological

Process for coupling biological substances by covalent bonds

The present invention relates to a conjugate of a specific ligand and a lectin, by covalent bonding. The product of the invention may, for example, be the conjugate of Concanavalin A and an antigen, a hapten, an antibody, a hormone or its receptor, an enzyme or its inhibitor or a lectin. Coupling being effected by means of glutaraldehyde or p-benzoquinone.

Conjugate obtained by coupling a lectin and a specific ligand,

A solution of an active protein substance and an inactive protein substance is reacted with a cross-linking agent, optionally in the presence of an inert carrier, under cross-linking conditions to produce articles comprising both active and inactive protein substances. The active protein substance comprises up to about 20 percent, e.g. from 1 to 20 percent by weight, based on the final weight of the total protein substance, whereas the cross-linking agent comprises from 0.5 to 8 percent by weight, based on the weight of the total treated mixture. The obtained articles are in the form of a solution or a suspension in aqueous medium, in the form of a film, in the form of a membrane, in the form of a fabric, in the form of a porous material, or in the form of a mass, such as granules, pills or tablets.

Immobilization of active protein by cross-linking to inactive protein

The invention relates to a magnetic gel which is useful for immunoezymatic determinations. Said gel consisting, for example, of polyacrylamideagarose and magnetic particles, coupled with an antibody, can be used for rapid determinations of the antigens contained in a biological liquid, the presence of magnetic particles permitting rapid separation of the gel from the reaction medium by means of a magnetic field and considerably shortening the time required for determinations.

Magnetic gel suitable to immunoenzymatic determinations

Growing community of inventors

Paris, France

Stratis Avrameas