Hamlet Protein A/s

Stig Victor Petersen

Katrine Hvid Ellegård

Svend Andreas Geleff

Jonatan Ahrens Dickow

Laila Thirup

The invention relates to a method for producing a solid transformation product of a substrate comprising the following steps: &#x2022;preparing a substrate of biomass comprising carbohydrates and proteinaceous matter that originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with carbohydrates and proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, &#x2022;mixing said substrate with a live microorganism or a combination of live microorganisms, which live microorganism or mixture of live microorganisms is not live yeast, and adding water in an amount which provides an initial incubation mixture having a water content from 30 to 70% by weight, and a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; &#x2022;incubating said initial incubation mixture for 1-240 hours at a temperature of 15-70&#xb0; C.; and thereafter recovering wet solid transformation product from the incubation mixture; further comprising that the incubating step is performed as a continuous plug-flow process in a vertical, non-agitated incubation tank with inlet means for said mixture and additives and outlet means for said solid transformation product.

Vertical plug-flow process for bio-conversion employing microorganisms

The invention relates to a method for the simultaneous production of a solid transformation product of the substrate and crude ethanol comprising the following steps: &#x2022;preparing a substrate from milled or flaked biomass comprising proteinaceous matter which originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, &#x2022;mixing said substrate with live yeast in a dry matter ratio of from 1:1 to 10,000:1 and adding water in an amount which provides a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; &#x2022;incubating said mixture for 1-48 hours at a temperature of about 20-60&#xb0; C.; and &#x2022;separating crude ethanol and wet solid transformation product from said mixture; further comprising that the incubation is performed as a continuous plug-flow process in a vertical, non-agitated, closed incubation tank with inlet means for said mixture and additives and outlet means for the solid transformation product and crude ethanol. The invention further relates to the products of this method as well as uses thereof.

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Beder, Denmark

Stig Victor Petersen