The State University of New York

Rutgers, the State University of New Jersey

Stewart N Loh

Adam R Blanden

David John Augeri

John A Gilleran

Darren R Carpizo

Spencer David Kimball

Xin Yu

Anthony F Bencivenga

Jeung-Hoi Ha

Alan Blayney

Mark C Butler

Tracy L Radley

Provided are compositions and methods for used in solubilizing, stabilizing and expressing proteins. The proteins are fusion proteins that contain a protein of interest. The fusion proteins contain segments of Ribose Binding Protein (RBP) or Maltose Binding Protein (MBP). The fusion proteins can have the RBP or MBP segments flanking the target protein, and the RBP or MBP segments can be in the fusion protein in the same orientation as they normally occur (except for being interrupted by the target protein) or the segments can be permuted. Novel segments of the RBP and MBP are provided and result in improved expression and/or solubility of the proteins. Some examples include one or a combination of two complete or partial Histidine tags. Some examples allow for the target protein to be separated from all or a part of the fusion protein such as by enzymatic or non-enzymatic cleavage.

Compositions and methods comprising permuted protein tags for facilitating overexpression, solubility, and purification of target proteins

Provided are compositions and methods for enhancing recombinant protein production. The compositions and methods involve use of Ribose Binding Protein (RBP) as a segment of a fusion polypeptide, whereby the RBP segment enhances production of the fusion protein. The fusion proteins contain the RBP sequentially in a single fusion protein with a polypeptide for which enhanced expression is desired. Recombinant expression vectors encoding the fusion proteins that contain and RBP segment are included, as are cells that contain the expression vectors. Methods for separating fusion proteins and for liberating a polypeptide segment that is part of the fusion protein are also provided.

Cleavable fusion tag for protein overexpression and purification

The invention provides compounds of formula (I): and salts thereof wherein ring A, R, HET, X, n, and Rhave any of the meanings described in the specification, as well as compositions comprising such compounds and salts, and methods for treating cancer using such compounds and salts. 

Hydrazone derivatives for the treatment of cancer

The invention provides complexes of Zn2+ of formulae (la) and (IIa) that are useful for treating cancer, as well as compositions and kits comprising such complexes. 

Zinc complexes of hydrazones and (thio)semicarbazones and their use for the treatment of cancer

The invention provides organic complexes of Znthat are useful for treating cancer, as well as compositions and kits comprising such complexes, and intermediate monomer compounds that are useful for the preparation of such complexes.

(Thio, oxo and seleno) semicarbazone complexes with zinc and their use for treating cancer

The invention provides compounds of formula I and II and salts thereof, wherein R, R, Y, R, and Rhave any of the meanings described in the specification, as well as compositions comprising such compounds and salts, and methods for treating cancer using such compounds and salts. 

(Thio, oxo, and seleno) semicarbazone derivatives and their use for treating cancer

The invention provides organic complexes of Znof formula (I) that are useful for treating cancer, as well as compositions and kits comprising such complexes, and intermediate monomer compounds that are useful for the preparation of such complexes. 

(Thio, oxo, and seleno) semicarbazone complexes with zinc and their use for treating cancer

The present invention is a two-domain, bi-functional fusion protein that functions as a molecular switch wherein the free energy released by the folding of a first domain of the fusion protein drives an unfolding of a second domain of the fusion protein, and vice versa. The molecular structure of the fusion protein is engineered so that, at any time, the folding of the first domain necessarily unfolds the other domain, and vice versa, thereby making the folded and unfolded states of the first and second domains mutually exclusive. This is accomplished by the insertion of ubiquitin insert protein into a surface loop of barnase target protein subject to the structural design criterion that the N-C terminal length of the ubiquitin insert protein is at least two-times greater than the C&#x3b1;-C&#x3b1; alpha-carbon-alpha-carbon length of the surface loop of the barnase target protein.

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Syracuse, NY, United States of America

Stewart N Loh